Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
911,132 |
A→G |
intergenic (‑83/+50) |
poxB ← / ← hcr |
pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 911,132 | 0 | A | G | 100.0%
| 194.8
/ NA
| 55 | intergenic (‑83/+50) | poxB/hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/29); total (26/29) |
ATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGA > NC_000913/911007‑911243
|
aTTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAAt > 1:264271/1‑139 (MQ=255)
gAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc < 1:295796/139‑1 (MQ=255)
ttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa < 2:264271/139‑1 (MQ=255)
tGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaaa > 1:1023373/1‑139 (MQ=255)
cGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGa < 2:1023373/139‑1 (MQ=255)
ataAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa < 1:741986/111‑1 (MQ=255)
ataAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa > 2:741986/1‑111 (MQ=255)
ataAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAatat > 1:1114075/1‑139 (MQ=255)
aGCTGCAACCGTTTATTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGATCATCTGACGGAGGGGGAAGGGATCGGAGAGAAAGGAGGCGCTAACGGTTATATAGCCCGATGAAAGGGATatcat < 1:922216/139‑1 (MQ=255)
gCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggg < 2:1114075/139‑1 (MQ=255)
aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa > 1:433690/1‑139 (MQ=255)
aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa > 1:1238846/1‑139 (MQ=255)
ccGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATa < 2:433690/139‑1 (MQ=255)
aTCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaa > 1:226429/1‑139 (MQ=255)
tctcCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa < 2:1238846/139‑1 (MQ=255)
ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt > 2:995811/1‑73 (MQ=255)
ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 1:995811/73‑1 (MQ=255)
gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGa > 1:22899/1‑139 (MQ=255)
aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGc > 1:1211171/1‑108 (MQ=255)
aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGc < 2:1211171/108‑1 (MQ=255)
gTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCTGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCg < 1:900406/130‑1 (MQ=255)
gTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCTGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCg > 2:900406/1‑130 (MQ=255)
aCAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat > 2:658084/1‑87 (MQ=255)
aCAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat < 1:658084/87‑1 (MQ=255)
gTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc < 2:226429/139‑1 (MQ=255)
tttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc < 2:1058320/139‑1 (MQ=255)
ttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa < 1:311044/139‑1 (MQ=255)
tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 2:1474496/47‑1 (MQ=255)
tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt > 1:1474496/1‑47 (MQ=255)
tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg < 1:407040/131‑1 (MQ=255)
tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg > 2:407040/1‑131 (MQ=255)
tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAg > 1:320526/1‑139 (MQ=255)
aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt > 2:160827/1‑46 (MQ=255)
aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 1:799991/46‑1 (MQ=255)
aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 1:547071/46‑1 (MQ=255)
aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 1:160827/46‑1 (MQ=255)
aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa < 2:1262908/56‑1 (MQ=255)
aGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa > 1:1262908/1‑56 (MQ=255)
ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATcccc < 2:1441770/112‑1 (MQ=255)
ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATcccc > 1:1441770/1‑112 (MQ=255)
ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg > 1:576747/1‑128 (MQ=255)
ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg < 2:576747/128‑1 (MQ=255)
cATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg < 1:331564/59‑1 (MQ=255)
cATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg > 2:331564/1‑59 (MQ=255)
tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa < 1:617934/62‑1 (MQ=255)
tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa > 2:617934/1‑62 (MQ=255)
tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa < 1:1099278/79‑1 (MQ=255)
tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa > 2:1099278/1‑79 (MQ=255)
cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt > 2:449637/1‑39 (MQ=255)
cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 1:449637/39‑1 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGa > 1:1366633/1‑139 (MQ=255)
agaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat < 1:231983/47‑1 (MQ=255)
agaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat > 2:231983/1‑47 (MQ=255)
aggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCAt > 2:642559/1‑47 (MQ=255)
aggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCAt < 1:642559/47‑1 (MQ=255)
|
ATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGA > NC_000913/911007‑911243
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
