Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
911,132 |
A→G |
intergenic (‑83/+50) |
poxB ← / ← hcr |
pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 911,132 | 0 | A | G | 100.0%
| 229.3
/ NA
| 64 | intergenic (‑83/+50) | poxB/hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/34); total (30/34) |
CGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGT > NC_000913/911005‑911256
|
cGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaa < 2:446186/139‑1 (MQ=255)
tCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa < 1:1300811/128‑1 (MQ=255)
tCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa > 2:1300811/1‑128 (MQ=255)
gAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTGCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc < 2:1158050/139‑1 (MQ=255)
aGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat < 1:1274988/139‑1 (MQ=255)
aGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat < 2:734090/139‑1 (MQ=255)
gCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCggg < 2:1421284/139‑1 (MQ=255)
gCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCggg < 2:595964/139‑1 (MQ=255)
aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat > 2:1556078/1‑133 (MQ=255)
aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat < 1:1556078/133‑1 (MQ=255)
ttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGc > 2:1328428/1‑139 (MQ=255)
tttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 1:468644/91‑1 (MQ=255)
tttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt > 2:468644/1‑91 (MQ=255)
ttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGAtt < 2:1299548/139‑1 (MQ=255)
aTGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa > 1:691514/1‑109 (MQ=255)
aTGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa < 2:691514/109‑1 (MQ=255)
tcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAAc > 2:106806/1‑139 (MQ=255)
tctcCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa > 1:1692937/1‑139 (MQ=255)
tctcCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa < 1:106806/139‑1 (MQ=255)
ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt < 2:814125/73‑1 (MQ=255)
ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt > 1:814125/1‑73 (MQ=255)
aaTGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCt > 1:1671739/1‑139 (MQ=255)
gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat < 1:1597508/97‑1 (MQ=255)
gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat > 2:1597508/1‑97 (MQ=255)
tAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat < 1:1725030/95‑1 (MQ=255)
tAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat > 2:1725030/1‑95 (MQ=255)
cGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat < 2:1224234/92‑1 (MQ=255)
cGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat > 1:1224234/1‑92 (MQ=255)
gTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCa < 1:360356/96‑1 (MQ=255)
gTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCa > 2:360356/1‑96 (MQ=255)
gTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAgg > 1:1192367/1‑139 (MQ=255)
tAACAAGTTTAGTTCATCTGACGGGGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGa > 2:371753/1‑139 (MQ=255)
tAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGa > 1:1023430/1‑139 (MQ=255)
cAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCa < 1:410448/139‑1 (MQ=255)
gTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc < 2:1192367/139‑1 (MQ=255)
tttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAA‑GAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGTAGCAGGCCa < 1:1162759/139‑1 (MQ=255)
ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAg > 1:1530052/1‑57 (MQ=255)
ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAg < 2:1530052/57‑1 (MQ=255)
aTCTGACGGGGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTa < 1:371753/139‑1 (MQ=255)
tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat > 1:184519/1‑73 (MQ=255)
tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATatcat < 2:184519/73‑1 (MQ=255)
tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTaa > 1:691463/1‑139 (MQ=255)
cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGc < 2:651659/124‑1 (MQ=255)
cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGc > 1:651659/1‑124 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc < 1:1573906/45‑1 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc > 2:1573906/1‑45 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGc < 1:205768/44‑1 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGc > 2:205768/1‑44 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCgag > 2:1152172/1‑86 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCgag < 1:1152172/86‑1 (MQ=255)
gggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGAt < 1:1046564/48‑1 (MQ=255)
gggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGAt > 2:1046564/1‑48 (MQ=255)
gATGGGAGAGAAAGGAGGTGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCg > 1:170850/1‑139 (MQ=255)
gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg < 2:984253/39‑1 (MQ=255)
gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg > 1:984253/1‑39 (MQ=255)
ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc > 2:1017161/1‑97 (MQ=255)
ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc < 1:1017161/97‑1 (MQ=255)
gagagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGt < 2:1023430/139‑1 (MQ=255)
gaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg < 2:1397910/98‑1 (MQ=255)
gaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg > 1:1397910/1‑98 (MQ=255)
aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAttt > 2:461155/1‑124 (MQ=255)
aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAttt < 1:461155/124‑1 (MQ=255)
aggCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg < 2:517011/81‑1 (MQ=255)
aggCGCTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg > 1:517011/1‑81 (MQ=255)
|
CGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAA‑GGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGT > NC_000913/911005‑911256
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
