Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
911,132 |
A→G |
intergenic (‑83/+50) |
poxB ← / ← hcr |
pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 911,132 | 0 | A | G | 100.0%
| 107.4
/ NA
| 31 | intergenic (‑83/+50) | poxB/hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/16); total (15/16) |
GATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATT > NC_000913/911006‑911266
|
gATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa < 1:1289660/139‑1 (MQ=255)
tcAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAAAAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggg < 1:557442/138‑1 (MQ=255)
aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa > 2:201822/1‑139 (MQ=255)
gtttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg < 2:1188648/139‑1 (MQ=255)
tttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAgg > 2:416132/1‑139 (MQ=255)
ggTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCg < 1:295355/139‑1 (MQ=255)
tctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa < 2:20315/121‑1 (MQ=255)
tctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa > 1:20315/1‑121 (MQ=255)
ctcCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACg > 1:305930/1‑79 (MQ=255)
ctcCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACg < 2:305930/79‑1 (MQ=255)
ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATcc > 2:970703/1‑139 (MQ=255)
gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg > 2:158532/1‑107 (MQ=255)
gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg < 1:158532/107‑1 (MQ=255)
aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa > 1:1056701/1‑65 (MQ=255)
aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa < 2:1056701/65‑1 (MQ=255)
aGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCa < 1:686736/137‑1 (MQ=255)
aGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCa > 2:686736/1‑137 (MQ=255)
ttAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa > 1:440926/1‑139 (MQ=255)
tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa < 1:657347/49‑1 (MQ=255)
tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa > 2:657347/1‑49 (MQ=255)
tGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGTACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAcc < 1:970703/139‑1 (MQ=255)
aGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGc < 1:201822/139‑1 (MQ=255)
gggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggg > 2:1177540/1‑68 (MQ=255)
gggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggg < 1:1177540/68‑1 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc > 1:836161/1‑45 (MQ=255)
ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc < 2:836161/45‑1 (MQ=255)
agaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAg < 1:416132/139‑1 (MQ=255)
gaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGc > 2:785325/1‑139 (MQ=255)
aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg < 2:199332/55‑1 (MQ=255)
aaaGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAg > 1:199332/1‑55 (MQ=255)
aggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCAtt > 2:1349267/1‑139 (MQ=255)
|
GATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATT > NC_000913/911006‑911266
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
