Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,431,882 |
T→C |
V945A (GTG→GCG) |
stfR → |
Rac prophage, putative tail fiber protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,431,882 | 0 | T | C | 100.0%
| 148.8
/ NA
| 42 | V945A (GTG→GCG) | stfR | Rac prophage, putative tail fiber protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/21); total (21/21) |
ACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTT > NC_000913/1431748‑1432005
|
accaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc < 1:738045/139‑1 (MQ=255)
tCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGc < 2:1057060/137‑1 (MQ=255)
tCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGc > 1:1057060/1‑137 (MQ=255)
tCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTg > 1:1114244/1‑139 (MQ=255)
tCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTg > 1:1297345/1‑139 (MQ=255)
aCCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt < 2:1239275/139‑1 (MQ=255)
ccGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCa < 2:1297345/139‑1 (MQ=255)
gCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCt > 1:68842/1‑139 (MQ=255)
tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc < 1:1238472/118‑1 (MQ=255)
tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc > 2:1238472/1‑118 (MQ=255)
ccGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCggg > 2:1113392/1‑136 (MQ=255)
ccGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCggg < 1:1113392/136‑1 (MQ=255)
ccGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCg > 1:385202/1‑139 (MQ=255)
gTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa < 1:1332483/139‑1 (MQ=255)
gTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa < 2:385202/139‑1 (MQ=255)
tATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGtctc < 2:1114244/139‑1 (MQ=255)
gATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt > 1:200595/1‑115 (MQ=255)
gATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt < 2:200595/115‑1 (MQ=255)
gggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATCTGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGaa > 2:1474651/1‑129 (MQ=255)
gggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGaa < 1:1474651/129‑1 (MQ=255)
gggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCa > 2:1356390/1‑139 (MQ=255)
ggCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGcc > 2:1200564/1‑97 (MQ=255)
ggCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGcc < 1:1200564/97‑1 (MQ=255)
gCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCg > 1:426726/1‑68 (MQ=255)
gCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCg < 2:426726/68‑1 (MQ=255)
ttGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCggg > 2:1290690/1‑102 (MQ=255)
ttGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCggg < 1:1290690/102‑1 (MQ=255)
gCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCAcagcg > 1:1429403/1‑139 (MQ=255)
cccGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCAcagcgccagc < 1:1356390/139‑1 (MQ=255)
cGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTc < 1:844388/81‑1 (MQ=255)
cGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTc > 2:844388/1‑81 (MQ=255)
aaaCTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa > 1:490215/1‑138 (MQ=255)
aaaCTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa < 2:490215/138‑1 (MQ=255)
tGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCca < 2:1114734/120‑1 (MQ=255)
tGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCca > 1:1114734/1‑120 (MQ=255)
cTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGAttt > 2:1130417/1‑139 (MQ=255)
aTCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa < 2:1097966/97‑1 (MQ=255)
aTCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa > 1:1097966/1‑97 (MQ=255)
aGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt > 1:843181/1‑55 (MQ=255)
aGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt < 2:843181/55‑1 (MQ=255)
aGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATAAAATCGCATAcc > 1:815950/1‑100 (MQ=255)
aGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATAAAATCGCATAcc < 2:815950/100‑1 (MQ=255)
|
ACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTT > NC_000913/1431748‑1432005
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
