Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,431,882	T→C	V945A (GTG→GCG)	stfR →	Rac prophage, putative tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,431,882	0	T	C	100.0%	183.5 / NA	50	V945A (GTG→GCG)	stfR	Rac prophage, putative tail fiber protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/25); total (25/25)

CACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACG  >  NC_000913/1431747‑1432012

caccaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGAt                                                                                                                                 >  2:36414/1‑139 (MQ=255)

caccaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGAt                                                                                                                                 >  2:380633/1‑139 (MQ=255)

          cTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATg                                                                                                                       <  1:156814/139‑1 (MQ=255)

               cAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTgg                                                                                                                  <  1:142107/139‑1 (MQ=255)

               cAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTgg                                                                                                                  >  2:142107/1‑139 (MQ=255)

                               cccGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc                                                                                                  <  1:352812/139‑1 (MQ=255)

                                   tGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAAc                                                                                              >  1:508887/1‑139 (MQ=255)

                                     gCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCt                                                                                            >  2:730064/1‑139 (MQ=255)

                                        aTCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGcc                                                                                         >  1:789006/1‑139 (MQ=255)

                                         tCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCa                                                                                        <  2:324372/139‑1 (MQ=255)

                                                 cGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCgg                                                                                <  2:379303/139‑1 (MQ=255)

                                                   ttCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCa                                                                                        <  1:280780/129‑1 (MQ=255)

                                                   ttCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCa                                                                                        >  2:280780/1‑129 (MQ=255)

                                                   ttCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGc                                                                              <  1:325279/139‑1 (MQ=255)

                                                   ttCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTCTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGc                                                                              >  1:442165/1‑139 (MQ=255)

                                                    tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGCAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGa                                                                                                             <  2:573395/107‑1 (MQ=255)

                                                    tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTTAGGCGCGATCCCTGATATGCGTGGCTGGa                                                                                                             >  1:573395/1‑107 (MQ=255)

                                                      cGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGt                                                                           <  1:36414/139‑1 (MQ=255)

                                                       gTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                          <  1:448026/139‑1 (MQ=255)

                                                       gTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                          <  1:730064/139‑1 (MQ=255)

                                                                tGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAg                                                                 >  2:309719/1‑139 (MQ=255)

                                                                  cccTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc                                                                                                                                <  2:92194/74‑1 (MQ=255)

                                                                  cccTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc                                                                                                                                >  1:92194/1‑74 (MQ=255)

                                                                  cccTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc                                                                                                  >  1:694532/1‑104 (MQ=255)

                                                                  cccTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc                                                                                                  <  2:694532/104‑1 (MQ=255)

                                                                  cccTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGa                                                               >  2:7491/1‑139 (MQ=255)

                                                                      gATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc                                                                                                  <  2:909568/100‑1 (MQ=255)

                                                                      gATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc                                                                                                  >  1:909568/1‑100 (MQ=255)

                                                                       aTGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTgg                                                                                                              >  1:837972/1‑87 (MQ=255)

                                                                       aTGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTgg                                                                                                              <  2:837972/87‑1 (MQ=255)

                                                                        tGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGa                                                         <  2:789006/139‑1 (MQ=255)

                                                                            ggggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt                                                                                   <  1:11134/109‑1 (MQ=255)

                                                                            ggggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt                                                                                   >  2:11134/1‑109 (MQ=255)

                                                                             gggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCa                                                    <  1:885147/139‑1 (MQ=255)

                                                                               gCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCAtt                                                  >  1:911554/1‑139 (MQ=255)

                                                                                 aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                          >  1:423287/1‑113 (MQ=255)

                                                                                 aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                          <  2:423287/113‑1 (MQ=255)

                                                                                 aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa                                                >  1:646011/1‑139 (MQ=255)

                                                                                 aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa                                                <  1:45925/139‑1 (MQ=255)

                                                                                 aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa                                                >  2:374033/1‑139 (MQ=255)

                                                                                  ggCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTgg                                                                                                                  <  1:833942/72‑1 (MQ=255)

                                                                                  ggCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTgg                                                                                                                  >  2:833942/1‑72 (MQ=255)

                                                                                      tttGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGc                                           <  1:380633/139‑1 (MQ=255)

                                                                                                   cTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAt                                                                                                         <  2:145933/64‑1 (MQ=255)

                                                                                                   cTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAt                                                                                                         >  1:145933/1‑64 (MQ=255)

                                                                                                       cccGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCAcagcgccagc                          <  1:7491/139‑1 (MQ=255)

                                                                                                                 gCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTa                >  1:342724/1‑139 (MQ=255)

                                                                                                                  cAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTAc               >  2:267637/1‑139 (MQ=255)

                                                                                                                              cGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGAc   <  1:267637/139‑1 (MQ=255)

                                                                                                                               gTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACg  >  1:508151/1‑139 (MQ=255)

CACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACG  >  NC_000913/1431747‑1432012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A