Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,431,882	T→C	V945A (GTG→GCG)	stfR →	Rac prophage, putative tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,431,882	0	T	C	100.0%	160.8 / NA	45	V945A (GTG→GCG)	stfR	Rac prophage, putative tail fiber protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/22); total (23/22)

CACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAAC  >  NC_000913/1431747‑1432017

caccaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGAt                                                                                                                                      <  1:412737/139‑1 (MQ=255)

 accaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc                                                                                                                                     <  2:1590454/139‑1 (MQ=255)

            aCCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCg                                                                                                                          >  1:1431750/1‑139 (MQ=255)

             cccAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGt                                                                                                                         <  1:1621436/139‑1 (MQ=255)

                aGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGc                                                                                                                      <  2:1284285/139‑1 (MQ=255)

                aGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGc                                                                                                                      >  2:1167034/1‑139 (MQ=255)

                 gTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCt                                                                                                                     >  1:1583777/1‑139 (MQ=255)

                   cGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTgg                                                                                                                   <  2:1262910/139‑1 (MQ=255)

                       gcACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGa                                                                                                               >  1:515480/1‑139 (MQ=255)

                        cACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGa                                                                                                                                <  2:1176006/121‑1 (MQ=255)

                        cACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGa                                                                                                                                >  1:1176006/1‑121 (MQ=255)

                            aaTCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAg                                                                                                          >  1:405310/1‑139 (MQ=255)

                                   tGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAAc                                                                                                   >  1:279897/1‑139 (MQ=255)

                                     gCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt                                                                                                             >  1:1464354/1‑127 (MQ=255)

                                     gCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt                                                                                                             <  2:1145205/127‑1 (MQ=255)

                                     gCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt                                                                                                             >  1:1145205/1‑127 (MQ=255)

                                     gCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTCGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt                                                                                                             <  2:1464354/127‑1 (MQ=255)

                                             aTACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTgg                                                                                         <  2:405310/139‑1 (MQ=255)

                                               aCCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTc                                                                                       >  1:575457/1‑139 (MQ=255)

                                                  gTTCCGTCTGGTTATGCCCTGATGCAGGGGCACGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCggg                                                                                    <  1:174674/139‑1 (MQ=255)

                                                   ttCCGTCTGGTTATGCCCTGATGCAGGGTCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTgg                                                                                         >  2:139034/1‑133 (MQ=255)

                                                   ttCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGc                                                                                   >  1:1113074/1‑139 (MQ=255)

                                                   ttCCGTCTGGTTATGCCCTGATGCAGCGTCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTgg                                                                                         <  1:139034/133‑1 (MQ=255)

                                                    tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGa                                                                                                                  >  2:1221918/1‑107 (MQ=255)

                                                    tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGa                                                                                                                  <  1:1221918/107‑1 (MQ=255)

                                                    tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGcc                                                                                  >  2:1582499/1‑139 (MQ=255)

                                                        tCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTAt                                                                              >  1:567646/1‑139 (MQ=255)

                                                        tCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTAt                                                                              >  1:1149957/1‑139 (MQ=255)

                                                         cTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTAtt                                                                             <  2:856339/139‑1 (MQ=255)

                                                                       aTGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGtc                                                                          <  2:1656207/128‑1 (MQ=255)

                                                                       aTGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGtc                                                                          >  1:1656207/1‑128 (MQ=255)

                                                                       aTGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAgg                                                               >  2:292237/1‑139 (MQ=255)

                                                                             gggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTc                                                                                       >  1:289759/1‑109 (MQ=255)

                                                                             gggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTc                                                                                       <  2:289759/109‑1 (MQ=255)

                                                                                 aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa                                                     >  1:695919/1‑139 (MQ=255)

                                                                                 aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa                                                     <  2:1289295/139‑1 (MQ=255)

                                                                                   gCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAAt                                                   <  1:724839/139‑1 (MQ=255)

                                                                                                       cccGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCAcagcgccagc                               <  1:1582499/139‑1 (MQ=255)

                                                                                                          gAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa                            <  1:1167034/139‑1 (MQ=255)

                                                                                                          gAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa                            <  2:567646/139‑1 (MQ=255)

                                                                                                            aaCTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATc                          <  2:1583777/139‑1 (MQ=255)

                                                                                                                        cTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGAttt              >  2:602071/1‑139 (MQ=255)

                                                                                                                              cGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGAc        <  2:515480/139‑1 (MQ=255)

                                                                                                                               gTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACg       >  1:203619/1‑139 (MQ=255)

                                                                                                                                   ggCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGCGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGaaaa   >  1:1136992/1‑139 (MQ=255)

                                                                                                                                    gcgcGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGCGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAAc  <  2:1136992/139‑1 (MQ=255)

CACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAAC  >  NC_000913/1431747‑1432017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A