Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,700,201 |
(T)5→6 |
coding (847/1593 nt) |
malX → |
maltose and glucose‑specific PTS enzyme IIB component and IIC component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,700,196 | 1 | . | T | 100.0%
| 129.9
/ NA
| 41 | G281V (GGT→GTT) | malX | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (22/19); total (22/19) |
CCGTTTGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGG‑T‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700060‑1700328
|
ccGTTTGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGG‑t‑t > 1:446069/1‑139 (MQ=255)
aTCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAgcg > 2:421945/1‑139 (MQ=255)
tCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAgcgc < 1:1265389/139‑1 (MQ=255)
tCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCAcgcg < 1:183860/139‑1 (MQ=255)
tggtggCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGATTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCACCAcgcg < 1:920212/137‑1 (MQ=255)
tggtggCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCAcgcg > 2:920212/1‑137 (MQ=255)
cTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTaa > 2:764011/1‑139 (MQ=255)
aCCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATg > 1:1356280/1‑139 (MQ=255)
aCCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATg > 1:1356281/1‑139 (MQ=255)
aCGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTg < 1:764011/139‑1 (MQ=255)
ggcggcACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGttt > 1:721934/1‑139 (MQ=255)
cggcACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTtct > 2:301299/1‑139 (MQ=255)
gcACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcg > 1:594522/1‑139 (MQ=255)
cACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcgg < 2:1314421/139‑1 (MQ=255)
aCGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATG‑CTGCGTTTCTcggcg < 2:1245142/139‑1 (MQ=255)
cGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCAc > 2:1055974/1‑98 (MQ=255)
cGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCAc < 1:1055974/98‑1 (MQ=255)
cAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGt < 1:301299/139‑1 (MQ=255)
aGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc < 2:612466/139‑1 (MQ=255)
aGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc < 1:421945/139‑1 (MQ=255)
tGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGt > 1:1454315/1‑139 (MQ=255)
cGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc > 1:779610/1‑133 (MQ=255)
cGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGc > 1:1270388/1‑139 (MQ=255)
cGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAAGTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc < 2:779610/133‑1 (MQ=255)
cggcgCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg > 1:51205/1‑111 (MQ=255)
cggcgCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg < 2:51205/111‑1 (MQ=255)
gcgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTg > 1:10222/1‑97 (MQ=255)
gcgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTg < 2:10222/97‑1 (MQ=255)
cgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcg < 1:965847/105‑1 (MQ=255)
cgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcg > 2:965847/1‑105 (MQ=255)
cgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCa > 1:1005015/1‑139 (MQ=255)
aGGCGCAATTGAGTTGCCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGaaa > 1:628282/1‑139 (MQ=255)
cAATTGAGTTACCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGt < 2:847807/88‑1 (MQ=255)
cAATTGAGTTACCCGACCACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGt > 1:847807/1‑88 (MQ=255)
ttGCGTTGCCCGACCACTCACGG‑TGTTTTCTGAAAGCTCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTTCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCAt < 2:594522/139‑1 (MQ=255)
ccACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt < 2:205846/93‑1 (MQ=255)
ccACTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt > 1:205846/1‑93 (MQ=255)
aCTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGt < 1:993968/70‑1 (MQ=255)
aCTCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGt > 2:993968/1‑70 (MQ=255)
tCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTctgct > 2:1237003/1‑139 (MQ=255)
tCACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTctgct < 1:1237003/139‑1 (MQ=255)
cACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 2:651338/86‑1 (MQ=255)
cACGGTT‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg > 1:651338/1‑86 (MQ=255)
|
CCGTTTGGTCTGCATCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGG‑T‑TTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700060‑1700328
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
