Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,700,201 |
(T)5→6 |
coding (847/1593 nt) |
malX → |
maltose and glucose‑specific PTS enzyme IIB component and IIC component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,700,196 | 1 | . | T | 100.0%
| 120.7
/ NA
| 38 | G281V (GGT→GTT) | malX | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (15/23); total (15/23) |
TCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGG‑‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700074‑1700328
|
tCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAgcgc < 2:588900/139‑1 (MQ=255)
tCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCAcgcg < 1:716790/139‑1 (MQ=255)
cTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTTTCTGAAAGCGCCAcgcg < 2:276187/139‑1 (MQ=255)
aaTTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCa < 2:1073586/139‑1 (MQ=255)
cGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGc > 2:103889/1‑139 (MQ=255)
cggcACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGt < 2:644439/107‑1 (MQ=255)
cggcACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGt > 1:644439/1‑107 (MQ=255)
cACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCCcgg < 1:683468/139‑1 (MQ=255)
gAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTg < 1:103889/139‑1 (MQ=255)
aaGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGc < 2:1056794/139‑1 (MQ=255)
aGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGcc < 1:692323/139‑1 (MQ=255)
tGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGTGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGt > 1:266362/1‑139 (MQ=255)
tGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGt > 1:59318/1‑139 (MQ=255)
gCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTcc < 2:826257/94‑1 (MQ=255)
gCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTcc > 1:826257/1‑94 (MQ=255)
gCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGAAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTg < 2:972824/139‑1 (MQ=255)
cAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTTCGTTTCTCGGCGGTCTGCCAGGTGCAGCg < 2:266334/139‑1 (MQ=255)
aaaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 2:407962/139‑1 (MQ=255)
aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGtt > 1:39919/1‑139 (MQ=255)
aaCCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGtt > 1:298208/1‑139 (MQ=255)
ccGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 2:440677/139‑1 (MQ=255)
aGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCAcgc > 1:1153224/1‑74 (MQ=255)
aGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCAcgc < 2:1153224/74‑1 (MQ=255)
ggcgCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTAt < 2:298208/139‑1 (MQ=255)
aCCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGG‑‑t < 1:1163288/42‑1 (MQ=255)
aCCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGG‑‑t > 2:1163288/1‑42 (MQ=255)
ggCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt > 2:1239266/1‑108 (MQ=255)
ggCGCAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 1:1239266/108‑1 (MQ=255)
cAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGc > 2:64856/1‑139 (MQ=255)
cAATTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGc > 2:913004/1‑139 (MQ=255)
aaTTGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGcc > 1:1161778/1‑139 (MQ=255)
ttGAGTTGCCCGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCAt > 1:1281769/1‑139 (MQ=255)
cGACCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAg < 2:933637/139‑1 (MQ=255)
aCCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGt < 2:39919/139‑1 (MQ=255)
aCCACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGt < 2:266362/139‑1 (MQ=255)
ccACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCaa < 2:720595/45‑1 (MQ=255)
ccACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCaa > 1:720595/1‑45 (MQ=255)
ccACTCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTc > 1:736610/1‑139 (MQ=255)
tCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCGGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTctgct < 2:897419/139‑1 (MQ=255)
tCACGGT‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTctgct < 1:984019/139‑1 (MQ=255)
|
TCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGG‑‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700074‑1700328
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
