Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,700,201 |
(T)5→6 |
coding (847/1593 nt) |
malX → |
maltose and glucose‑specific PTS enzyme IIB component and IIC component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,700,196 | 1 | . | T | 100.0%
| 65.3
/ NA
| 22 | G281V (GGT→GTT) | malX | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (9/13); total (9/13) |
TCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑G‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700074‑1700328
|
tCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAgcgc < 1:119022/139‑1 (MQ=255)
gtggCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTc > 2:140927/1‑139 (MQ=255)
gCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCtt > 1:487132/1‑139 (MQ=255)
cATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCttt > 2:795965/1‑139 (MQ=255)
aTTCGCTTTACCGACGCGGGCGGCACGCAGGAAGTCTACGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCaa < 1:795965/139‑1 (MQ=255)
cGACCCAGGGGGCAAGCAGGGAGTCTGCGGTCAAACCGTCAGCGGTGCACGCACCATCCACCAGGCGCAATTTACTTGGGCTACCACTCACGTT‑TTTTTCTTAAAGCGCCGTGCGTTTCCTCT‑GCCAGGGAAAAtactc > 1:258839/1‑135 (MQ=255)
cGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg < 2:487132/139‑1 (MQ=255)
cAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGt < 1:140927/139‑1 (MQ=255)
gTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCa > 1:64966/1‑139 (MQ=255)
gCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTg < 1:307511/139‑1 (MQ=255)
ccGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 2:613902/139‑1 (MQ=255)
ccGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 2:90971/139‑1 (MQ=255)
gcgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 1:62598/130‑1 (MQ=255)
gcgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg > 2:62598/1‑130 (MQ=255)
ccAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGa > 2:504119/1‑139 (MQ=255)
aGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 1:336015/109‑1 (MQ=255)
aGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGACTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt > 2:336015/1‑109 (MQ=255)
cAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 1:711836/104‑1 (MQ=255)
cAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt > 2:711836/1‑104 (MQ=255)
aaTTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt < 1:676718/108‑1 (MQ=255)
aaTTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt > 2:676718/1‑108 (MQ=255)
ttGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCAt < 2:64966/139‑1 (MQ=255)
tCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTctgct < 2:797849/139‑1 (MQ=255)
|
TCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑G‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700074‑1700328
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
