Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,964	C→T	317 (TAG→TAA)	cbl ←	ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,964	0	C	T	100.0%	138.3 / NA	43	317 (TAG→TAA)	cbl	ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/22); total (21/22)

GATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTT  >  NC_000913/2059832‑2060092

gATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATc                                                                                                                            <  1:981412/139‑1 (MQ=255)

    tgCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                        >  2:403523/1‑139 (MQ=255)

       cTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAAt                                                                                                                     <  2:477933/139‑1 (MQ=255)

               cGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTcc                                                                                                             >  2:151119/1‑139 (MQ=255)

                    ccTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTc                                                                                                        >  2:1267230/1‑139 (MQ=255)

                        tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                    <  2:365935/139‑1 (MQ=255)

                         gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                                   <  1:403523/139‑1 (MQ=255)

                                  tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                    >  1:195518/1‑129 (MQ=255)

                                  tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                    <  2:195518/129‑1 (MQ=255)

                                  tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCa                                                                                          >  1:754100/1‑139 (MQ=255)

                                    ggTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAcc                                                                                        >  2:1165858/1‑139 (MQ=255)

                                      tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTACACTGCTTTCCATCACCTg                                                                                      >  1:231264/1‑139 (MQ=255)

                                            ggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCtt                                                                                >  2:458030/1‑139 (MQ=255)

                                             gcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                               <  1:936022/139‑1 (MQ=255)

                                                           cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                 <  1:151119/139‑1 (MQ=255)

                                                           cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                 <  2:754100/139‑1 (MQ=255)

                                                           cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                                 <  1:1267230/139‑1 (MQ=255)

                                                               tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCt                                                                                       <  2:1110857/113‑1 (MQ=255)

                                                               tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCt                                                                                       >  1:1110857/1‑113 (MQ=255)

                                                                     tGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                            <  2:143159/134‑1 (MQ=255)

                                                                     tGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                            >  1:143159/1‑134 (MQ=255)

                                                                       gTTCGAGTCCAGTCAGCGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                             <  2:950368/115‑1 (MQ=255)

                                                                       gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                             >  1:950368/1‑115 (MQ=255)

                                                                          cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTACACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                  <  2:231264/139‑1 (MQ=255)

                                                                           gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTatat                                                                                                                             >  1:1247176/1‑63 (MQ=255)

                                                                           gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTatat                                                                                                                             <  2:1247176/63‑1 (MQ=255)

                                                                                  gTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCa                                          >  2:176429/1‑139 (MQ=255)

                                                                                           gCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGcc                                 <  1:458030/139‑1 (MQ=255)

                                                                                            ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                  <  2:870/121‑1 (MQ=255)

                                                                                            ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                  >  1:870/1‑121 (MQ=255)

                                                                                                         aaGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGc                   >  1:1378569/1‑139 (MQ=255)

                                                                                                            cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                    >  1:492375/1‑55 (MQ=255)

                                                                                                            cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                    <  2:492375/55‑1 (MQ=255)

                                                                                                            cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                                   >  1:631716/1‑120 (MQ=255)

                                                                                                            cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                                   <  2:631716/120‑1 (MQ=255)

                                                                                                                           tgATGTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                            <  2:181025/79‑1 (MQ=255)

                                                                                                                           ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                            >  1:181025/1‑80 (MQ=255)

                                                                                                                           ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGc                   >  1:767303/1‑121 (MQ=255)

                                                                                                                           ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGc                   <  2:767303/121‑1 (MQ=255)

                                                                                                                           ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTg                 >  1:148883/1‑123 (MQ=255)

                                                                                                                           ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTg                 <  2:148883/123‑1 (MQ=255)

                                                                                                                           ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCtt  <  1:1490660/138‑1 (MQ=255)

                                                                                                                           ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCtt  >  2:1490660/1‑138 (MQ=255)

GATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTT  >  NC_000913/2059832‑2060092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A