Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,964	C→T	317 (TAG→TAA)	cbl ←	ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,964	0	C	T	100.0%	165.6 / NA	51	317 (TAG→TAA)	cbl	ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/25); total (26/25)

TTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAG  >  NC_000913/2059828‑2060095

ttAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTta                                                                                                                                   <  2:623992/139‑1 (MQ=255)

        tgCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                           >  1:426701/1‑139 (MQ=255)

                        ccTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTc                                                                                                           >  2:632979/1‑139 (MQ=255)

                                 ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATa                                                                                                                          >  2:628412/1‑115 (MQ=255)

                                 ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATa                                                                                                                          <  1:628412/115‑1 (MQ=255)

                                           aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGc                                                                                        >  2:918555/1‑139 (MQ=255)

                                                  cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTatat                                                                                                                                >  1:767082/1‑92 (MQ=255)

                                                  cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTatat                                                                                                                                <  2:767082/92‑1 (MQ=255)

                                                  cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                           >  1:860921/1‑97 (MQ=255)

                                                  cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                           <  2:860921/97‑1 (MQ=255)

                                                  cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGa                                                                                 >  1:710861/1‑139 (MQ=255)

                                                        gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCt                                                                           <  2:426701/139‑1 (MQ=255)

                                                                 tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACa                                                                  >  1:120645/1‑139 (MQ=255)

                                                                   tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGt                                                                >  2:94061/1‑139 (MQ=255)

                                                                   tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGt                                                                >  1:932193/1‑139 (MQ=255)

                                                                    gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                               <  1:343588/139‑1 (MQ=255)

                                                                    gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                               <  2:932193/139‑1 (MQ=255)

                                                                    gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                               <  1:897414/139‑1 (MQ=255)

                                                                       aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCtt                                                                                                            <  2:208333/91‑1 (MQ=255)

                                                                       aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCtt                                                                                                            >  1:208333/1‑91 (MQ=255)

                                                                       aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTg                                                            >  2:945560/1‑139 (MQ=255)

                                                                          ggTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAt                                                         >  1:917760/1‑139 (MQ=255)

                                                                           gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                        >  2:512848/1‑139 (MQ=255)

                                                                            ttCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTa                                                       <  2:710861/139‑1 (MQ=255)

                                                                            ttCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTa                                                       <  2:917760/139‑1 (MQ=255)

                                                                              cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                       >  2:592458/1‑89 (MQ=255)

                                                                              cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                       <  1:592458/89‑1 (MQ=255)

                                                                              cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                     <  1:945560/139‑1 (MQ=255)

                                                                               gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                               <  1:530854/128‑1 (MQ=255)

                                                                               gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                               >  2:530854/1‑128 (MQ=255)

                                                                                     agttaaaggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                           <  2:122789/56‑1 (MQ=255)

                                                                                     aGTCAAAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                           >  1:122789/1‑62 (MQ=255)

                                                                                       tCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                                  <  2:258768/101‑1 (MQ=255)

                                                                                       tCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                                  >  1:258768/1‑101 (MQ=255)

                                                                                        cAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGa                                           <  2:921924/139‑1 (MQ=255)

                                                                                                ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                                      >  2:537663/1‑136 (MQ=255)

                                                                                                ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                                      <  1:537663/136‑1 (MQ=255)

                                                                                                ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCAGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCa                                   >  2:537125/1‑139 (MQ=255)

                                                                                                  aaaTTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGa                                 <  1:512848/139‑1 (MQ=255)

                                                                                                     ttCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                     <  1:805886/132‑1 (MQ=255)

                                                                                                     ttCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                                     >  2:805886/1‑132 (MQ=255)

                                                                                                                cccGCTTTTATAGCTGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGTTTGATATCCt                                                                           >  2:614211/1‑83 (MQ=255)

                                                                                                                cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCt                                                                           <  1:614211/83‑1 (MQ=255)

                                                                                                                  cGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAg                 <  1:918555/139‑1 (MQ=255)

                                                                                                                         ataGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCt          <  2:120645/139‑1 (MQ=255)

                                                                                                                          taGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTc         >  1:256433/1‑139 (MQ=255)

                                                                                                                               ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGcc                                    <  1:647299/107‑1 (MQ=255)

                                                                                                                               ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGcc                                    >  2:647299/1‑107 (MQ=255)

                                                                                                                               ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAg                                  <  2:308465/109‑1 (MQ=255)

                                                                                                                               ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAg                                  >  1:308465/1‑109 (MQ=255)

                                                                                                                                 aTTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAg  >  2:100229/1‑139 (MQ=255)

TTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAG  >  NC_000913/2059828‑2060095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A