Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,085,831 A→G L411P (CTG→CCG)  plaP ← putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,8310AG99.3% 500.8 / ‑6.0 138L411P (CTG→CCG) plaPputrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (71/66);  total (71/67)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

ATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGC  >  NC_000913/2085703‑2085965
                                                                                                                                 |                                                                                                                                      
atTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAAc                                                                                                                              <  1:113721/139‑1 (MQ=255)
   aTGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           <  1:275402/139‑1 (MQ=255)
         cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           >  1:113019/1‑133 (MQ=255)
         cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           <  2:113019/133‑1 (MQ=255)
         cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcgc                                                                                                                     >  2:497034/1‑139 (MQ=255)
              tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                     <  2:633922/118‑1 (MQ=255)
              tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                     >  1:633922/1‑118 (MQ=255)
              tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           <  2:466895/128‑1 (MQ=255)
              tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcaccac                                                                                                                <  2:225221/139‑1 (MQ=255)
               cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                        >  2:39234/1‑130 (MQ=255)
               cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                        <  1:39234/130‑1 (MQ=255)
               cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcaca                                                                                                               >  2:889306/1‑139 (MQ=255)
                 tACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAGCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                             >  2:892124/1‑139 (MQ=255)
                     gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                             >  2:1236625/1‑135 (MQ=255)
                     gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                             <  1:1236625/135‑1 (MQ=255)
                      cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                                >  1:599147/1‑115 (MQ=255)
                      cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                                <  2:599147/115‑1 (MQ=255)
                      cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                         <  2:620994/122‑1 (MQ=255)
                      cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  2:1437926/1‑139 (MQ=255)
                      cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  1:83440/1‑139 (MQ=255)
                       ggAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       <  1:30676/139‑1 (MQ=255)
                        gAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           >  1:457215/1‑118 (MQ=255)
                        gAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAg                                                                                                      >  2:179708/1‑139 (MQ=255)
                           ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                             <  2:1365973/129‑1 (MQ=255)
                           ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                             >  1:1365973/1‑129 (MQ=255)
                           ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   <  1:668732/139‑1 (MQ=255)
                           ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   <  2:503627/139‑1 (MQ=255)
                             ggATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                 <  2:692880/139‑1 (MQ=255)
                              gATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAg                                                                                                >  1:219275/1‑139 (MQ=255)
                                 tACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATa                                                                                             <  1:858843/139‑1 (MQ=255)
                                   cGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           >  1:973308/1‑107 (MQ=255)
                                      aaGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                        <  2:847501/139‑1 (MQ=255)
                                        gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                         >  2:876235/1‑104 (MQ=255)
                                        gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                         <  1:876235/104‑1 (MQ=255)
                                         ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      >  2:588084/1‑106 (MQ=255)
                                         ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                     >  1:1318772/1‑139 (MQ=255)
                                           ctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         >  1:166004/1‑117 (MQ=255)
                                           ctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         <  2:166004/117‑1 (MQ=255)
                                           ctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                               <  2:807015/127‑1 (MQ=255)
                                           ctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                               >  1:807015/1‑127 (MQ=255)
                                                    gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGc                                                                          >  1:1481689/1‑139 (MQ=255)
                                                      aaGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGt                                                                        >  1:624145/1‑139 (MQ=255)
                                                        gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         >  2:104186/1‑105 (MQ=255)
                                                        gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         <  1:104186/105‑1 (MQ=255)
                                                        gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCt                                                                      >  1:150266/1‑139 (MQ=255)
                                                             gTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                 <  2:83440/139‑1 (MQ=255)
                                                                  tCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      <  2:1498343/81‑1 (MQ=255)
                                                                  tCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      >  1:1498343/1‑81 (MQ=255)
                                                                  tCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                 <  2:1325085/134‑1 (MQ=255)
                                                                  tCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                 >  1:1325085/1‑134 (MQ=255)
                                                                  tCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCtt                                                            >  1:1277063/1‑139 (MQ=255)
                                                                  tCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCtt                                                            >  2:1302941/1‑139 (MQ=255)
                                                                      aCCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCa                                                        >  2:1060735/1‑139 (MQ=255)
                                                                      aCCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCa                                                        <  1:179708/139‑1 (MQ=255)
                                                                       ccGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                     <  2:1108123/109‑1 (MQ=255)
                                                                       ccGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                     >  1:1108123/1‑109 (MQ=255)
                                                                       ccGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       <  1:1437926/139‑1 (MQ=255)
                                                                       ccGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       <  2:132174/139‑1 (MQ=255)
                                                                          aTAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGa                                                                                                    >  2:584389/1‑91 (MQ=255)
                                                                          aTAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGa                                                                                                    <  1:584389/91‑1 (MQ=255)
                                                                          aTAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGa                                                    >  2:768170/1‑139 (MQ=255)
                                                                                 cccAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        >  1:474737/1‑80 (MQ=255)
                                                                                 cccAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                        <  2:474737/80‑1 (MQ=255)
                                                                                 cccAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGaa                                             <  1:768170/139‑1 (MQ=255)
                                                                                 cccAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGaa                                             >  1:1421716/1‑139 (MQ=255)
                                                                                      aTCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         >  2:1264493/1‑74 (MQ=255)
                                                                                      aTCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                         <  1:1264493/74‑1 (MQ=255)
                                                                                         aGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCgaga                                     <  1:497034/139‑1 (MQ=255)
                                                                                           a‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      <  1:1483937/56‑1 (MQ=255)
                                                                                           a‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                      >  2:1483937/1‑56 (MQ=255)
                                                                                           a‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   >  2:613190/1‑75 (MQ=255)
                                                                                           a‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                   <  1:613190/75‑1 (MQ=255)
                                                                                           a‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCt                                                             >  2:364739/1‑113 (MQ=255)
                                                                                           a‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       >  1:151018/1‑119 (MQ=255)
                                                                                                aGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                               <  1:496851/139‑1 (MQ=255)
                                                                                                aGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                               <  2:219275/139‑1 (MQ=255)
                                                                                                 gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                         <  1:1482256/48‑1 (MQ=255)
                                                                                                 gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                     <  1:1065540/84‑1 (MQ=255)
                                                                                                 gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       >  2:1067301/1‑114 (MQ=255)
                                                                                                 gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                              >  2:598454/1‑139 (MQ=255)
                                                                                                 gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                              <  1:1060735/139‑1 (MQ=255)
                                                                                                  aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                           >  2:1485078/1‑45 (MQ=255)
                                                                                                      aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                         <  1:584606/43‑1 (MQ=255)
                                                                                                       ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGt                                                                   >  1:860121/1‑96 (MQ=255)
                                                                                                       ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGtt                        >  2:319349/1‑139 (MQ=255)
                                                                                                        tGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                 <  1:892988/65‑1 (MQ=255)
                                                                                                            cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       <  1:711911/103‑1 (MQ=255)
                                                                                                            cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       >  2:711911/1‑103 (MQ=255)
                                                                                                               tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       >  1:1242065/1‑100 (MQ=255)
                                                                                                               tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                       <  2:1242065/100‑1 (MQ=255)
                                                                                                               tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAg                <  2:152362/139‑1 (MQ=255)
                                                                                                                 ttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       >  1:914104/1‑50 (MQ=255)
                                                                                                                 ttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       <  2:914104/50‑1 (MQ=255)
                                                                                                                         tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                       <  2:1455088/42‑1 (MQ=255)
                                                                                                                         tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccac      <  2:1277063/139‑1 (MQ=255)
                                                                                                                           aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                         >  1:838566/1‑70 (MQ=255)
                                                                                                                           aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                         <  2:838566/70‑1 (MQ=255)
                                                                                                                            cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTAca                                                               <  2:939444/79‑2 (MQ=255)
                                                                                                                            cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAg   >  1:1301212/1‑139 (MQ=255)
                                                                                                                             ccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAgc  >  1:512277/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                      
ATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGA‑CCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGC  >  NC_000913/2085703‑2085965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A