Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 750 51.0 2595496 93.6% 2429384 106.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,085,831 A→G L411P (CTG→CCG)  plaP ← putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,8310AG100.0% 391.7 / NA 109L411P (CTG→CCG) plaPputrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (54/55);  total (54/55)

Only 100 of 109 total aligned reads displayed.

AAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGC  >  NC_000913/2085696‑2085958
                                                                                                                                          |                                                                                                                               
aaaGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                              <  1:1088589/139‑1 (MQ=255)
  aGATGATTATGCTACGTCTTCGTACTGCGGAACCGGCTTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCa                                                                                                                            >  2:530625/1‑139 (MQ=255)
          aTGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                    <  2:351871/139‑1 (MQ=255)
             cTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTc                                                                                                                   >  2:1283709/1‑139 (MQ=255)
             cTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCAcc                                                                                                                          <  2:158025/130‑1 (MQ=255)
             cTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCAcc                                                                                                                          >  1:158025/1‑130 (MQ=255)
               aCGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                               <  2:741880/139‑1 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcgc                                                                                                              >  2:747996/1‑139 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcgc                                                                                                              >  2:122171/1‑139 (MQ=255)
                cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcgc                                                                                                              >  2:449417/1‑139 (MQ=255)
                  tCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCa                                                                                                                  >  2:1286150/1‑133 (MQ=255)
                     tCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcaccac                                                                                                         >  2:530049/1‑139 (MQ=255)
                            gCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                              <  1:625228/111‑1 (MQ=255)
                            gCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                            <  2:917762/129‑1 (MQ=255)
                            gCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                            >  1:917762/1‑129 (MQ=255)
                            gCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                  <  2:1252014/139‑1 (MQ=255)
                             cGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                 >  1:49578/1‑139 (MQ=255)
                                 aCCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGTAAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                              >  2:861400/1‑139 (MQ=255)
                                  ccGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                            <  2:573801/139‑1 (MQ=255)
                                  ccGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                            <  2:1119887/139‑1 (MQ=255)
                                  ccGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                            <  1:1087978/139‑1 (MQ=255)
                                    ggATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                          <  1:449417/139‑1 (MQ=255)
                                     gATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                      >  1:317594/1‑126 (MQ=255)
                                     gATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                      <  2:317594/126‑1 (MQ=255)
                                     gATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAg                                                                                         >  2:540356/1‑139 (MQ=255)
                                     gATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAg                                                                                         >  1:513551/1‑139 (MQ=255)
                                      aTTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                        <  1:540356/139‑1 (MQ=255)
                                             aaGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                 <  1:140197/139‑1 (MQ=255)
                                               gCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                     <  1:1126118/133‑1 (MQ=255)
                                               gCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                     >  2:1126118/1‑133 (MQ=255)
                                                ctctTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                     >  2:1126840/1‑132 (MQ=255)
                                                ctctTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                     <  1:1126840/132‑1 (MQ=255)
                                                     g‑‑gTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcaca                                                                                                        >  1:1217337/1‑108 (MQ=255)
                                                           aCGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                    >  1:749061/1‑92 (MQ=255)
                                                           aCGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                      >  2:228192/1‑106 (MQ=255)
                                                           aCGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                      <  1:228192/106‑1 (MQ=255)
                                                           aCGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                          <  2:810918/118‑1 (MQ=255)
                                                           aCGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                          >  1:810918/1‑118 (MQ=255)
                                                           aCGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACCGt                                                                                                                    <  2:749061/92‑1 (MQ=255)
                                                            cGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                              >  1:460977/1‑81 (MQ=255)
                                                            cGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                              <  2:460977/81‑1 (MQ=255)
                                                            cGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAg                                                                    <  2:386928/139‑1 (MQ=255)
                                                             gCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                 <  2:467585/109‑1 (MQ=255)
                                                             gCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                 >  1:467585/1‑109 (MQ=255)
                                                               aaGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGt                                                                 >  2:204264/1‑139 (MQ=255)
                                                                      gT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                          >  1:735201/1‑139 (MQ=255)
                                                                       t‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTAc                                                         <  1:922633/139‑1 (MQ=255)
                                                                         aaaTCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                        <  1:747996/139‑1 (MQ=255)
                                                                            tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCtt                                                     >  1:278280/1‑139 (MQ=255)
                                                                               gACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTtctc                                                  <  2:735201/139‑1 (MQ=255)
                                                                                aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCa                                                 >  1:1047187/1‑139 (MQ=255)
                                                                                 ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                >  1:858556/1‑139 (MQ=255)
                                                                                     tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                          <  1:1236522/93‑1 (MQ=255)
                                                                                     tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                          >  2:1236522/1‑93 (MQ=255)
                                                                                         agcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAg                                        <  2:513551/139‑1 (MQ=255)
                                                                                           cccAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                            <  2:350161/85‑1 (MQ=255)
                                                                                           cccAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                            >  1:350161/1‑85 (MQ=255)
                                                                                             cAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACt                                    >  1:751707/1‑139 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcacca                                                                                                          >  2:288147/1‑61 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcacca                                                                                                          <  1:288147/61‑1 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                  <  1:1231256/69‑1 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                  >  2:1231256/1‑69 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                     <  2:593629/98‑1 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                     >  1:593629/1‑98 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGc                                                       >  1:141487/1‑112 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGc                                                       <  2:141487/112‑1 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                            >  1:320089/1‑139 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                            >  1:623516/1‑139 (MQ=255)
                                                                                                     aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                            >  1:65540/1‑139 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                 <  1:964588/81‑1 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                     <  1:505300/93‑1 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                     >  2:505300/1‑93 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACgg                                              >  2:379746/1‑116 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACgg                                              <  1:379746/116‑1 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAg                                        <  1:227847/122‑1 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAg                                        >  2:227847/1‑122 (MQ=255)
                                                                                                          gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                       >  1:328735/1‑139 (MQ=255)
                                                                                                           aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                      <  2:858556/139‑1 (MQ=255)
                                                                                                           aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                      <  2:202291/139‑1 (MQ=255)
                                                                                                              cATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                        <  2:319485/134‑1 (MQ=255)
                                                                                                               aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                         >  1:308529/1‑36 (MQ=255)
                                                                                                               aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                         <  2:308529/36‑1 (MQ=255)
                                                                                                               aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                              <  2:173672/63‑1 (MQ=255)
                                                                                                               aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                              >  1:173672/1‑63 (MQ=255)
                                                                                                               aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                             <  1:114225/80‑1 (MQ=255)
                                                                                                               aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                             >  2:114225/1‑80 (MQ=255)
                                                                                                               aTTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGt                  >  2:419271/1‑139 (MQ=255)
                                                                                                                ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCa                                         <  1:1139375/115‑1 (MQ=255)
                                                                                                                ttGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCa                                         >  2:1139375/1‑115 (MQ=255)
                                                                                                                 tGAACTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCCAGATGACCGACAGGTTa                >  2:743072/1‑139 (MQ=255)
                                                                                                                     cTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGa            <  2:65540/139‑1 (MQ=255)
                                                                                                                        tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                            <  2:1208491/56‑1 (MQ=255)
                                                                                                                        tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                            >  1:1208491/1‑56 (MQ=255)
                                                                                                                        tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                <  2:1116986/100‑1 (MQ=255)
                                                                                                                        tcttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                >  1:1116986/1‑100 (MQ=255)
                                                                                                                          ttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTg       >  2:616024/1‑139 (MQ=255)
                                                                                                                          ttcCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTg       <  1:419271/139‑1 (MQ=255)
                                                                                                                            ccAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGaa     <  1:616024/139‑1 (MQ=255)
                                                                                                                               ggTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGc  <  1:228368/139‑1 (MQ=255)
                                                                                                                                  tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                        <  2:813345/114‑1 (MQ=255)
                                                                                                                                          |                                                                                                                               
AAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTG‑‑GTGACGCAAGCCAGGT‑AAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGC  >  NC_000913/2085696‑2085958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A