Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,085,831	A→G	L411P (CTG→CCG)	plaP ←	putrescine importer, low affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,085,831	0	A	G	100.0%	167.9 / NA	48	L411P (CTG→CCG)	plaP	putrescine importer, low affinity
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/23); total (25/23)

TGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCA  >  NC_000913/2085701‑2085963

tgatTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCa                                                                                                                              <  1:257635/139‑1 (MQ=255)

     aTGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGt                                                                                                                         <  2:538297/139‑1 (MQ=255)

                tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                   >  2:654554/1‑118 (MQ=255)

                tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                   <  1:654554/118‑1 (MQ=255)

                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                           <  2:570831/134‑1 (MQ=255)

                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                           >  1:570831/1‑134 (MQ=255)

                        cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                      <  2:275142/139‑1 (MQ=255)

                          gAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCCTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAg                                                                                                    >  1:196578/1‑139 (MQ=255)

                             ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                      <  2:709994/118‑1 (MQ=255)

                             ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                      >  1:709994/1‑118 (MQ=255)

                             ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                 >  1:461385/1‑139 (MQ=255)

                             ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                 <  2:215018/139‑1 (MQ=255)

                                 aTTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                             <  2:461385/139‑1 (MQ=255)

                                                      gCAAGCCAGTTAAATCAGACCGAAAGCAGCCCAGATCAGA‑CCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                       <  2:699510/139‑1 (MQ=255)

                                                                    tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                    >  1:433831/1‑81 (MQ=255)

                                                                    tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                    <  2:433831/81‑1 (MQ=255)

                                                                        aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCa                                                      >  2:512604/1‑139 (MQ=255)

                                                                         ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                     <  1:370253/139‑1 (MQ=255)

                                                                         ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                     <  2:196578/139‑1 (MQ=255)

                                                                             tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGAt                                                 >  2:242166/1‑139 (MQ=255)

                                                                                  gcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGa                                            >  2:657721/1‑139 (MQ=255)

                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                   <  2:104411/41‑1 (MQ=255)

                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGgcg                                                                                                                                   >  1:104411/1‑41 (MQ=255)

                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                          <  2:292408/82‑1 (MQ=255)

                                                                                             aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                          >  1:292408/1‑82 (MQ=255)

                                                                                                cAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                    >  2:155469/1‑53 (MQ=255)

                                                                                                cAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                    <  1:155469/53‑1 (MQ=255)

                                                                                                cAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGAcc                              >  1:24005/1‑139 (MQ=255)

                                                                                                 aGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                             <  2:667803/139‑1 (MQ=255)

                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                 <  1:680675/54‑1 (MQ=255)

                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGcac                                                                                                                 >  2:680675/1‑54 (MQ=255)

                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                                   <  1:377196/68‑1 (MQ=255)

                                                                                                  gAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                                   >  2:377196/1‑68 (MQ=255)

                                                                                                   aaCCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                           <  1:400588/139‑1 (MQ=255)

                                                                                                         tGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTa                     >  1:259398/1‑139 (MQ=255)

                                                                                                         tGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTa                     >  2:299310/1‑139 (MQ=255)

                                                                                                         tGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTa                     >  1:173847/1‑139 (MQ=255)

                                                                                                                    ccAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCGCACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGaa          <  1:512604/139‑1 (MQ=255)

                                                                                                                     cAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                             <  1:59503/87‑1 (MQ=255)

                                                                                                                     cAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                             >  2:59503/1‑87 (MQ=255)

                                                                                                                     cAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAAc         <  1:299310/139‑1 (MQ=255)

                                                                                                                       ggTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGc       >  1:739987/1‑139 (MQ=255)

                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                           >  2:637226/1‑114 (MQ=255)

                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                           <  1:637226/114‑1 (MQ=255)

                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca  >  2:516386/1‑139 (MQ=255)

                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca  >  1:799320/1‑139 (MQ=255)

                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca  >  2:597101/1‑139 (MQ=255)

                                                                                                                            aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGCTAACGAAAGTGAACGccacca  >  2:472201/1‑139 (MQ=255)

TGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCA  >  NC_000913/2085701‑2085963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A