Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,706,482	C→T	G215S (GGC→AGC)	lepA ←	back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,706,482	0	C	T	100.0%	152.5 / NA	47	G215S (GGC→AGC)	lepA	back‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/25); total (22/25)

GAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTG  >  NC_000913/2706351‑2706617

gAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTa                                                                                                                                  <  1:467326/139‑1 (MQ=255)

  gccagccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAAt                                                                                                                                >  1:829787/1‑139 (MQ=255)

   ccagccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               <  2:155098/139‑1 (MQ=255)

    cagccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATAc                                                                                                                              >  1:259691/1‑139 (MQ=255)

       ccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGa                                                                                                                           >  1:892769/1‑139 (MQ=255)

       ccTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGa                                                                                                                           >  1:4452/1‑139 (MQ=255)

        cTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGAt                                                                                                                          <  2:259691/139‑1 (MQ=255)

                ccACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGaaa                                                                                                                  >  2:379491/1‑139 (MQ=255)

                  acaTTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAaaca                                                                                                                <  1:379491/139‑1 (MQ=255)

                     tttCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAaac                                                                                                                 <  1:107593/135‑1 (MQ=255)

                     tttCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAaac                                                                                                                 >  2:107593/1‑135 (MQ=255)

                       tcagttcagtGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGcc                                                                                                           <  2:442342/139‑1 (MQ=255)

                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGAt                                                                                                                          <  2:642657/115‑1 (MQ=255)

                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGAt                                                                                                                          >  1:642657/1‑115 (MQ=255)

                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACACAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGcc                                                                                                           <  2:86333/130‑1 (MQ=255)

                                tGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACACAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGcc                                                                                                           >  1:86333/1‑130 (MQ=255)

                                          cTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        >  2:778291/1‑139 (MQ=255)

                                          cTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        <  2:829787/139‑1 (MQ=255)

                                             tttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAg                                                                                     <  1:778291/139‑1 (MQ=255)

                                              ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               <  1:1183011/96‑1 (MQ=255)

                                              ttCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               >  2:1183011/1‑96 (MQ=255)

                                                       aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               <  1:734933/87‑1 (MQ=255)

                                                       aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATa                                                                                                                               >  2:734933/1‑87 (MQ=255)

                                                       aaGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTa                                                                           <  1:784120/139‑1 (MQ=255)

                                                           tGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGc                                                                       <  2:1220022/139‑1 (MQ=255)

                                                                 gACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCaa                                                                 >  2:841398/1‑139 (MQ=255)

                                                                    ggTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  2:1044588/139‑1 (MQ=255)

                                                                              ataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                    <  2:892769/139‑1 (MQ=255)

                                                                              ataGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGa                                                    <  2:4452/139‑1 (MQ=255)

                                                                                       cccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  2:1270194/120‑1 (MQ=255)

                                                                                       cccGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              >  1:1270194/1‑120 (MQ=255)

                                                                                                 aTGACTTTCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGaa                                 >  1:299102/1‑139 (MQ=255)

                                                                                                      tttCACTTTGTCGCCCTTACGCAGTGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                            <  1:841398/139‑1 (MQ=255)

                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        <  1:336644/79‑1 (MQ=255)

                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCAt                                                                                        >  2:336644/1‑79 (MQ=255)

                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  1:37110/105‑1 (MQ=255)

                                                                                                      tttCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              >  2:37110/1‑105 (MQ=255)

                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACgg                                                                                                                            <  1:1120683/41‑1 (MQ=255)

                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACgg                                                                                                                            >  2:1120683/1‑41 (MQ=255)

                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                            <  2:589409/137‑1 (MQ=255)

                                                                                                        tCACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTcg                            >  1:589409/1‑137 (MQ=255)

                                                                                                         cACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                           <  2:418441/105‑1 (MQ=255)

                                                                                                         cACTTTGTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGcc                                                           >  1:418441/1‑105 (MQ=255)

                                                                                                               gTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              <  1:9059/96‑1 (MQ=255)

                                                                                                               gTCGCCCTTACGCAGGGTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACgg                                                              >  2:9059/1‑96 (MQ=255)

                                                                                                                              ggTGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc    >  1:48530/1‑139 (MQ=255)

                                                                                                                                tGCTGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTg  >  2:998644/1‑139 (MQ=255)

GAGCCAGCCTACTTCGCCACATTTCAGTTCAGTGCGGTCAACCTGTTTCGGCGTGAAGATGCCCAGACGGTCGGCGTTATAGGTCTGCCCGGTACTCATGACTTTCACTTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTG  >  NC_000913/2706351‑2706617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A