Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,200,881	G→A	A51V (GCC→GTC)	xisE ←	e14 prophage, putative excisionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,200,881	0	G	A	100.0%	166.9 / NA	53	A51V (GCC→GTC)	xisE	e14 prophage, putative excisionase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/25); total (28/25)

AGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAG  >  NC_000913/1200754‑1201014

aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGc                                                                                                                            >  2:411096/1‑139 (MQ=255)

aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGc                                                                                                                            >  1:577386/1‑139 (MQ=255)

 gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                           <  2:1140569/139‑1 (MQ=255)

  cGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCAc                                                                                                                          >  2:1065475/1‑139 (MQ=255)

    gATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTc                                                                                                                        >  1:67284/1‑139 (MQ=255)

       ataAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGcc                                                                                                                     >  1:820748/1‑139 (MQ=255)

                 gACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGcc                                                                                                           >  2:133235/1‑139 (MQ=255)

                 gACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGcc                                                                                                           <  1:133235/139‑1 (MQ=255)

                    tGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                           <  1:25786/120‑1 (MQ=255)

                    tGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCAAGCa                                                                                                                           >  2:25786/1‑120 (MQ=255)

                     gAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGt                                                                                                       >  1:654103/1‑139 (MQ=255)

                      aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGtt                                                                                                      >  2:728488/1‑139 (MQ=255)

                          attattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCAt                                                                                                  <  1:904721/139‑1 (MQ=255)

                                   aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTATCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                         <  1:178866/139‑1 (MQ=255)

                                   aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACg                                                                                                                             >  2:737312/1‑103 (MQ=255)

                                   aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACg                                                                                                                             <  1:737312/103‑1 (MQ=255)

                                   aCGCGGTCTGGCTGCAATTACTCAATATCCGTTGCAAAGGTAATGAGTCATTTTTCTTGCTTACAGGTGATACCAATTCCCCAATTAAATAGACGACCCCACGCACTCGCGATAACCTGCCTTCTTTCACGGCCGGtcg                                                                                         >  1:513010/1‑137 (MQ=255)

                                     gcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                       <  2:654103/139‑1 (MQ=255)

                                        gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    >  2:477080/1‑139 (MQ=255)

                                        gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaa                                                                                    >  2:1081335/1‑139 (MQ=255)

                                         tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                       >  1:72167/1‑135 (MQ=255)

                                         tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                       <  2:72167/135‑1 (MQ=255)

                                         tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                   >  1:54717/1‑139 (MQ=255)

                                          cTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                  <  1:993644/139‑1 (MQ=255)

                                               tGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAGCCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                             <  1:1398561/139‑1 (MQ=255)

                                               tGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                             <  1:728488/139‑1 (MQ=255)

                                                   aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                           <  1:411878/89‑1 (MQ=255)

                                                   aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                           >  2:411878/1‑89 (MQ=255)

                                                    ttACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                        <  1:411096/139‑1 (MQ=255)

                                                     tACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCtt                                                                       >  2:1373149/1‑139 (MQ=255)

                                                            aaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                     <  2:789818/134‑1 (MQ=255)

                                                            aaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                     >  1:789818/1‑134 (MQ=255)

                                                                  ttGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                          <  2:555828/139‑1 (MQ=255)

                                                                            aaTGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGaca                                                <  2:891790/139‑1 (MQ=255)

                                                                                   cATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                         <  1:416692/139‑1 (MQ=255)

                                                                                    aTTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgga                                        >  2:1164373/1‑139 (MQ=255)

                                                                                                   aGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCAt                                                                                                  <  1:61531/66‑1 (MQ=255)

                                                                                                   aGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCAt                                                                                                  >  2:61531/1‑66 (MQ=255)

                                                                                                    ggTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAtt                        <  1:477080/139‑1 (MQ=255)

                                                                                                       gtTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAttttt                     >  1:1222607/1‑139 (MQ=255)

                                                                                                        ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                    >  2:287503/1‑139 (MQ=255)

                                                                                                          aCCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcag                  >  2:1163956/1‑139 (MQ=255)

                                                                                                               cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                       >  1:556657/1‑65 (MQ=255)

                                                                                                               cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                       <  2:556657/65‑1 (MQ=255)

                                                                                                               cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                           <  2:241385/93‑1 (MQ=255)

                                                                                                               cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                           >  1:241385/1‑93 (MQ=255)

                                                                                                               cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGcc             <  2:590710/139‑1 (MQ=255)

                                                                                                               cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGcc             >  2:1074418/1‑139 (MQ=255)

                                                                                                               cTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGcc             >  2:400364/1‑139 (MQ=255)

                                                                                                                 ccccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa           <  2:67284/139‑1 (MQ=255)

                                                                                                                 ccccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa           <  1:1164373/139‑1 (MQ=255)

                                                                                                                 ccccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa           <  1:1081335/139‑1 (MQ=255)

                                                                                                                          actCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag  >  2:1216419/1‑139 (MQ=255)

AGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAG  >  NC_000913/1200754‑1201014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A