Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,200,881	G→A	A51V (GCC→GTC)	xisE ←	e14 prophage, putative excisionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,200,881	0	G	A	100.0%	146.2 / NA	47	A51V (GCC→GTC)	xisE	e14 prophage, putative excisionase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/23); total (24/23)

AAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCA  >  NC_000913/1200753‑1201012

aaGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACg                                                                                                                           <  1:13052/139‑1 (MQ=255)

  gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                         <  1:681179/139‑1 (MQ=255)

  gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                         <  2:744641/139‑1 (MQ=255)

  gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                         <  1:1276631/139‑1 (MQ=255)

  gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTATGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                         >  2:292218/1‑139 (MQ=255)

      atatAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCg                                                                                                                     >  2:687594/1‑139 (MQ=255)

      atatAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCg                                                                                                                     >  1:41012/1‑139 (MQ=255)

       tataAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGc                                                                                                                    <  1:232378/139‑1 (MQ=255)

                gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCgg                                                                                                           >  1:1047490/1‑139 (MQ=255)

                gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCgg                                                                                                           >  1:68196/1‑139 (MQ=255)

                       aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                         >  1:54432/1‑118 (MQ=255)

                       aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCa                                                                                                                         <  2:54432/118‑1 (MQ=255)

                                    aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCgg                                                                                                           <  2:702068/119‑1 (MQ=255)

                                    aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCgg                                                                                                           >  1:702068/1‑119 (MQ=255)

                                    aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                       >  1:3378/1‑139 (MQ=255)

                                     cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGa                                                                                      >  1:572357/1‑139 (MQ=255)

                                      gcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                     <  2:530877/139‑1 (MQ=255)

                                         gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                     >  2:1090114/1‑136 (MQ=255)

                                         gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                     <  1:1090114/136‑1 (MQ=255)

                                          tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                 >  1:674440/1‑139 (MQ=255)

                                                tGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                           <  2:68196/139‑1 (MQ=255)

                                                    aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                     <  1:268870/125‑1 (MQ=255)

                                                    aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                     >  2:268870/1‑125 (MQ=255)

                                                     ttACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                      <  2:41012/139‑1 (MQ=255)

                                                          cAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCa                                                                 <  1:709213/139‑1 (MQ=255)

                                                            aaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAAc                                                                                                              >  1:82847/1‑92 (MQ=255)

                                                            aaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAAc                                                                                                              <  2:82847/92‑1 (MQ=255)

                                                                 cGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                          <  2:572357/139‑1 (MQ=255)

                                                                                        tttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTggagga                                   >  2:237799/1‑139 (MQ=255)

                                                                                                         ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                <  2:862693/77‑1 (MQ=255)

                                                                                                         ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                >  1:862693/1‑77 (MQ=255)

                                                                                                         ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                >  1:554764/1‑109 (MQ=255)

                                                                                                         ttACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                <  2:554764/109‑1 (MQ=255)

                                                                                                          tACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTca                 >  1:440306/1‑139 (MQ=255)

                                                                                                          tACCAACTCCCCAACTAACTCCACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTca                 <  2:622253/139‑1 (MQ=255)

                                                                                                           aCCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcag                >  2:186446/1‑139 (MQ=255)

                                                                                                           aCCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAATACTTCTGTATTTTTcag                >  1:1246067/1‑139 (MQ=255)

                                                                                                             cAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagca              <  2:1246067/139‑1 (MQ=255)

                                                                                                                  ccccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa         <  1:237799/139‑1 (MQ=255)

                                                                                                                  ccccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa         >  2:1076028/1‑139 (MQ=255)

                                                                                                                   cccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCg                                                                                           <  2:308490/56‑1 (MQ=255)

                                                                                                                   cccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCg                                                                                           >  1:308490/1‑56 (MQ=255)

                                                                                                                   cccAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCACCAGCCCAt        >  1:1244610/1‑139 (MQ=255)

                                                                                                                           actCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCa  <  1:317930/137‑1 (MQ=255)

                                                                                                                           actCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCa  >  2:317930/1‑137 (MQ=255)

                                                                                                                            ctCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                           >  1:177995/1‑63 (MQ=255)

                                                                                                                            ctCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                           <  2:177995/63‑1 (MQ=255)

                                                                                                                             tCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                      <  2:272999/67‑1 (MQ=255)

AAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCA  >  NC_000913/1200753‑1201012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A