Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,758	C→T	461 (TAG→TAA)	dosC ←	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,758	0	C	T	100.0%	165.2 / NA	51	461 (TAG→TAA)	dosC	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/27); total (24/27)

AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGA  >  NC_000913/1565627‑1565892

aaCATCACTTCATCATTTTCGTTAATTAACACCGCACCCTTCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                                                 <  1:5375/139‑1 (MQ=255)

          catcatTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCa                                                                                                                       <  2:1183347/139‑1 (MQ=255)

                  tCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCaa                                                                                                               >  2:304461/1‑139 (MQ=255)

                    gTTTATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATTGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                             <  2:470467/139‑1 (MQ=255)

                    gTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                             <  1:548996/139‑1 (MQ=255)

                    gTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                             <  1:304461/139‑1 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCagag                                                                                                                    >  2:1192566/1‑118 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCagag                                                                                                                    <  1:1192566/118‑1 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                               >  1:347531/1‑139 (MQ=255)

                                     ccATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCt                                                                                            >  1:661354/1‑139 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAGCACGGTTTCTACCTCGTCttt                                                                                          >  1:841166/1‑139 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                          <  1:271685/139‑1 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                          >  2:1123218/1‑139 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                          <  2:274508/139‑1 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                          >  1:534321/1‑139 (MQ=255)

                                         catATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                        <  2:805298/139‑1 (MQ=255)

                                                tGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGatat                                                                                 >  1:612008/1‑139 (MQ=255)

                                                 gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                                >  2:1417442/1‑139 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                             >  1:1430334/1‑109 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                             <  2:1430334/109‑1 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATAc                                                                               >  1:178673/1‑139 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATAc                                                                               >  2:754567/1‑139 (MQ=255)

                                                      aGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                        >  1:1315607/1‑126 (MQ=255)

                                                      aGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                        <  2:1315607/126‑1 (MQ=255)

                                                               aaaaaaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgt                                                                  <  2:893890/139‑2 (MQ=255)

                                                                       gCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGCCGATATACAGAGCTTCATCGGCTATTTGa                                                          >  1:226793/1‑139 (MQ=255)

                                                                             ggcggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                    <  1:1377569/139‑1 (MQ=255)

                                                                                 gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                                <  2:178673/139‑1 (MQ=255)

                                                                                 gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                                <  2:612008/139‑1 (MQ=255)

                                                                                   aTTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                                >  2:211783/1‑137 (MQ=255)

                                                                                   aTTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                                <  1:211783/137‑1 (MQ=255)

                                                                                       tCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                                                 <  2:629229/52‑1 (MQ=255)

                                                                                       tCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                                                 >  1:629229/1‑52 (MQ=255)

                                                                                         cGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                             <  2:1334937/70‑1 (MQ=255)

                                                                                         cGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                             >  1:1334937/1‑70 (MQ=255)

                                                                                             tCGGTTAGCTTCATGATTACCTCTGCAGCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                    <  2:347531/139‑1 (MQ=255)

                                                                                               ggTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGa                                  <  2:534321/139‑1 (MQ=255)

                                                                                                                ccTCTGCATCCTGGCGCATTTAAAGACTGGCCTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcggc                 >  1:487640/1‑139 (MQ=255)

                                                                                                                   ctGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAac                                                                                                              <  1:907867/43‑1 (MQ=255)

                                                                                                                   ctGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAac                                                                                                              >  2:907867/1‑43 (MQ=255)

                                                                                                                   ctGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATgg                     <  2:1304734/132‑1 (MQ=255)

                                                                                                                   ctGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATgg                     >  1:1304734/1‑132 (MQ=255)

                                                                                                                      cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAac                                                                                                              <  2:972524/40‑1 (MQ=255)

                                                                                                                      cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAac                                                                                                              >  1:972524/1‑40 (MQ=255)

                                                                                                                      cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                    <  2:1153803/98‑1 (MQ=255)

                                                                                                                      cATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                    >  1:1153803/1‑98 (MQ=255)

                                                                                                                        tCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                    <  2:274252/96‑1 (MQ=255)

                                                                                                                        tCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                    >  1:274252/1‑96 (MQ=255)

                                                                                                                           tGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcgg                  >  2:117997/1‑127 (MQ=255)

                                                                                                                           tGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcgg                  <  1:117997/127‑1 (MQ=255)

                                                                                                                               gcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTga  <  2:487640/139‑1 (MQ=255)

AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGA  >  NC_000913/1565627‑1565892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A