Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,758	C→T	461 (TAG→TAA)	dosC ←	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,758	0	C	T	100.0%	138.8 / NA	43	461 (TAG→TAA)	dosC	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/23); total (20/23)

AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAA  >  NC_000913/1565627‑1565888

gagacagcTTCATCATTTTCGGTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                                              <  1:176580/132‑1 (MQ=255)

                  tCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCTTTATCCCCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGC‑CATTTTAAGGCTTGGCTTTCCagttgtctc                                                                                                           >  2:176580/1‑132 (MQ=255)

                  tCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCaa                                                                                                           >  1:113473/1‑139 (MQ=255)

                                cGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCt                                                                                             >  2:160010/1‑139 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  1:1038713/1‑139 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  1:20220/1‑139 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  1:1160247/1‑139 (MQ=255)

                                   aCCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCagag                                                                                                                <  1:344310/117‑1 (MQ=255)

                                   aCCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCagag                                                                                                                >  2:344310/1‑117 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                      <  2:113473/139‑1 (MQ=255)

                                            atTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTGGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                 <  1:160010/139‑1 (MQ=255)

                                                 gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                            >  2:1294649/1‑139 (MQ=255)

                                                 gCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGAtata                                                                            >  2:897146/1‑139 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAaca                                                                                                         >  2:1164194/1‑109 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAaca                                                                                                         <  1:1164194/109‑1 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATAc                                                                           >  1:153903/1‑139 (MQ=255)

                                                               aaaaaaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  1:1294649/139‑1 (MQ=255)

                                                               aaaaaaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  2:518319/139‑1 (MQ=255)

                                                                       gCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           <  1:906270/102‑1 (MQ=255)

                                                                       gCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                           >  2:906270/1‑102 (MQ=255)

                                                                            cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAg                                                 >  2:695147/1‑139 (MQ=255)

                                                                             ggcggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                <  2:1160247/139‑1 (MQ=255)

                                                                               cggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGgcg                                              <  1:897146/139‑1 (MQ=255)

                                                                                 gcATTATCCGCATCGGTTAGCTTCCTGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                            <  1:99617/139‑1 (MQ=255)

                                                                                 gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                            <  1:714671/139‑1 (MQ=255)

                                                                                 gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                            <  1:695147/139‑1 (MQ=255)

                                                                                      aTCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAg                                       <  2:561754/139‑1 (MQ=255)

                                                                                            aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGa                                 >  1:236751/1‑139 (MQ=255)

                                                                                             tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              >  2:907358/1‑109 (MQ=255)

                                                                                             tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  1:907358/109‑1 (MQ=255)

                                                                                             tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                <  2:261744/139‑1 (MQ=255)

                                                                                             tCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                <  2:1038713/139‑1 (MQ=255)

                                                                                                gTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGAc                             <  2:20220/139‑1 (MQ=255)

                                                                                                        cATGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAAc                     <  2:153903/139‑1 (MQ=255)

                                                                                                          tGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                    >  2:1269923/1‑74 (MQ=255)

                                                                                                          tGATTACCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                    <  1:1269923/74‑1 (MQ=255)

                                                                                                               aCCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                              >  2:814729/1‑91 (MQ=255)

                                                                                                               aCCTCTGCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGCCTTTTGGCGATATACAGAGCTTCATCgg                                                              <  1:814729/91‑1 (MQ=255)

                                                                                                                     gCATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGa        <  1:808502/139‑1 (MQ=255)

                                                                                                                      cATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                      >  2:301871/1‑60 (MQ=255)

                                                                                                                      cATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                      <  1:301871/60‑1 (MQ=255)

                                                                                                                      cATCCTGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGAt       >  1:1081607/1‑139 (MQ=255)

                                                                                                                           tGGCGCATTTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGaaa  >  2:157610/1‑139 (MQ=255)

AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGAC‑TGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAA  >  NC_000913/1565627‑1565888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A