Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,758	C→T	461 (TAG→TAA)	dosC ←	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,758	0	C	T	100.0%	153.4 / NA	47	461 (TAG→TAA)	dosC	diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/26); total (21/26)

AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAG  >  NC_000913/1565627‑1565893

aaCATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAATAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGAc                                                                                                                                  <  2:1262292/139‑1 (MQ=255)

                     ttaattaaCACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAacac                                                                                                             >  2:1732678/1‑139 (MQ=255)

                                cGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCt                                                                                                  >  2:745873/1‑139 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                                >  2:1581392/1‑139 (MQ=255)

                                  cACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCg                                                                                                >  2:1109243/1‑139 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGTCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           <  1:1643450/139‑1 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                              <  1:143935/120‑1 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                              >  2:143935/1‑120 (MQ=255)

                                       atcatATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           <  1:1665215/139‑1 (MQ=255)

                                         catATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                         <  1:1109243/139‑1 (MQ=255)

                                            atTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      <  1:745873/139‑1 (MQ=255)

                                            atTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      <  2:1847480/139‑1 (MQ=255)

                                            atTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      <  2:423614/139‑1 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGa                                                                                                                                   >  1:1936484/1‑88 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGa                                                                                                                                   <  2:1936484/88‑1 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                              >  1:2042821/1‑109 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                              <  2:2042821/109‑1 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           <  1:327711/128‑1 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                           >  2:327711/1‑128 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      <  2:269169/133‑1 (MQ=255)

                                                  cTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      >  1:269169/1‑133 (MQ=255)

                                                      aGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACagag                                                                            >  1:1641065/1‑139 (MQ=255)

                                                       gggCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACagacc                                                                           >  1:1218650/1‑137 (MQ=255)

                                                            gggAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCAt                                                                      >  2:1841769/1‑139 (MQ=255)

                                                             ggAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATc                                                                     <  1:10651/139‑1 (MQ=255)

                                                                   aaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGa                                                                                                                                   <  2:1450140/71‑1 (MQ=255)

                                                                   aaaTGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGa                                                                                                                                   >  1:1450140/1‑71 (MQ=255)

                                                                             ggcggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                     <  1:1732678/139‑1 (MQ=255)

                                                                             ggcggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                     <  2:25063/139‑1 (MQ=255)

                                                                                 gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCATAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                                 <  1:1872355/139‑1 (MQ=255)

                                                                                            aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                   <  2:1562468/110‑1 (MQ=255)

                                                                                            aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                   >  1:1562468/1‑110 (MQ=255)

                                                                                               ggTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGa                                   <  1:1581392/139‑1 (MQ=255)

                                                                                                               aCCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcgg                   <  1:1841769/139‑1 (MQ=255)

                                                                                                                    tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAg                                                      <  2:280262/99‑1 (MQ=255)

                                                                                                                    tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAg                                                      >  1:280262/1‑99 (MQ=255)

                                                                                                                    tGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCg              >  2:729763/1‑139 (MQ=255)

                                                                                                                           tGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGc                                                                  <  2:1490174/80‑1 (MQ=255)

                                                                                                                           tGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGc                                                                  >  1:1490174/1‑80 (MQ=255)

                                                                                                                             gcgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                   <  2:934541/77‑1 (MQ=255)

                                                                                                                             gcgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                   >  1:934541/1‑77 (MQ=255)

                                                                                                                              cgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      <  2:1304220/57‑1 (MQ=255)

                                                                                                                              cgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                      >  1:1304220/1‑57 (MQ=255)

                                                                                                                              cgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATc                                                                     <  1:817222/74‑1 (MQ=255)

                                                                                                                              cgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATc                                                                     >  2:817222/1‑74 (MQ=255)

                                                                                                                              cgcATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTg    >  2:118940/1‑139 (MQ=255)

                                                                                                                                cATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag  <  1:947923/139‑1 (MQ=255)

AACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAG  >  NC_000913/1565627‑1565893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A