Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
758 |
59.3 |
2864096 |
93.9% |
2689386 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,385,869 |
(A)7→6 |
coding (10/972 nt) |
yfbL → |
putative M28A family peptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,385,863 | 0 | A | . | 100.0%
| 130.6
/ NA
| 32 | coding (4/972 nt) | yfbL | putative M28A family peptidase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (18/14); total (18/14) |
TAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCG > NC_000913/2385735‑2385996
|
taaataaaCTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATc < 1:88701/139‑1 (MQ=255)
ctctTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACt > 1:991401/1‑139 (MQ=255)
tGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTttatta > 1:1411746/1‑115 (MQ=255)
tGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTttatta < 2:1411746/115‑1 (MQ=255)
aTCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGatta > 2:325221/1‑139 (MQ=255)
tGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTg < 1:1290845/96‑1 (MQ=255)
tGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTg > 2:1290845/1‑96 (MQ=255)
ataaAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCt > 1:525534/1‑139 (MQ=255)
tatCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTa < 1:63010/100‑1 (MQ=255)
tatCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTa > 2:63010/1‑100 (MQ=255)
gAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCtttt > 1:447975/1‑75 (MQ=255)
gAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCtttt < 2:447975/75‑1 (MQ=255)
aaaCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTAcc > 1:820233/1‑139 (MQ=255)
aCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAc > 1:697103/1‑114 (MQ=255)
aCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAc < 2:697103/114‑1 (MQ=255)
aCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt < 2:1020155/125‑1 (MQ=255)
aCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt > 1:1020155/1‑125 (MQ=255)
tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTttat < 1:1413068/69‑1 (MQ=255)
tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTttat > 2:1413068/1‑69 (MQ=255)
gATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGc < 2:525534/139‑1 (MQ=255)
gATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGc > 2:1282979/1‑139 (MQ=255)
tCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGa > 1:787752/1‑131 (MQ=255)
tCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGa < 2:787752/131‑1 (MQ=255)
aacaacGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCa > 2:20504/1‑106 (MQ=255)
aacaacGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCa < 1:20504/106‑1 (MQ=255)
cGCAACCCATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAg > 2:430843/1‑139 (MQ=255)
cccATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGc > 2:331547/1‑124 (MQ=255)
cccATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGc < 1:331547/124‑1 (MQ=255)
cccATTAATAATTTGCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAAtt > 2:527454/1‑139 (MQ=255)
gCATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTc > 2:806650/1‑139 (MQ=255)
cATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg < 1:325221/139‑1 (MQ=255)
cATATG‑AAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg < 2:991401/139‑1 (MQ=255)
|
TAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCG > NC_000913/2385735‑2385996
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
