Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
819,747 |
G→A |
*151* (TAG→TAA) |
moaE → |
molybdopterin synthase, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 819,747 | 0 | G | A | 100.0%
| 91.8
/ NA
| 29 | *151* (TAG→TAA) | moaE | molybdopterin synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/15); total (14/15) |
CGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCT > NC_000913/819614‑819851
|
cGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAAttttt > 2:833817/1‑139 (MQ=255)
aaGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg < 2:759925/139‑1 (MQ=255)
aaGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg < 2:787732/139‑1 (MQ=255)
ccGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCg < 1:833817/139‑1 (MQ=255)
gATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCg > 1:439414/1‑139 (MQ=255)
tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg > 2:362999/1‑139 (MQ=255)
tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg > 1:420780/1‑139 (MQ=255)
ctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGc > 1:198028/1‑139 (MQ=255)
aaCCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAAttt > 1:372903/1‑139 (MQ=255)
cgcgCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCAcc > 1:335038/1‑139 (MQ=255)
gcgcACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAAttt > 1:224371/1‑134 (MQ=255)
gcgcACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAAttt < 2:224371/134‑1 (MQ=255)
gcACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt < 1:362999/139‑1 (MQ=255)
gcACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt < 2:372903/139‑1 (MQ=255)
gcgcGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg < 1:746160/87‑1 (MQ=255)
gcgcGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg > 2:746160/1‑87 (MQ=255)
cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCa < 1:325690/124‑1 (MQ=255)
cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCa > 2:325690/1‑124 (MQ=255)
gAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGca > 2:18071/1‑139 (MQ=255)
ggCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGc < 1:256900/79‑1 (MQ=255)
ggCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGc > 2:256900/1‑79 (MQ=255)
gCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAAtttttt < 2:839834/56‑1 (MQ=255)
gCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAAtttttt > 1:839834/1‑56 (MQ=255)
gAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCa < 2:335038/139‑1 (MQ=255)
gAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCa < 1:18071/139‑1 (MQ=255)
aGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGt < 2:198028/139‑1 (MQ=255)
cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCa < 2:34246/133‑1 (MQ=255)
cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCa > 1:34246/1‑133 (MQ=255)
gggAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCt < 2:167594/139‑1 (MQ=255)
|
CGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCT > NC_000913/819614‑819851
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
