Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	819,747	G→A	151 (TAG→TAA)	moaE →	molybdopterin synthase, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	819,747	0	G	A	100.0%	162.5 / NA	51	151 (TAG→TAA)	moaE	molybdopterin synthase, large subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/26); total (25/26)

TTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTAT  >  NC_000913/819617‑819866

ttGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGt                                                                                                                 >  1:1188696/1‑139 (MQ=255)

   aaGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg                                                                                                              <  2:36679/139‑1 (MQ=255)

   aaGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg                                                                                                              <  1:275467/139‑1 (MQ=255)

        ggtcAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGa                                                                                                         <  1:1096381/136‑1 (MQ=255)

        gggCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGa                                                                                                         <  2:251423/139‑1 (MQ=255)

                        aTTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGt                                                                                         >  2:1137922/1‑139 (MQ=255)

                        aTTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGt                                                                                         >  1:1273205/1‑139 (MQ=255)

                          tATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTcc                                                                                       >  1:92277/1‑139 (MQ=255)

                            tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg                                                                                                              >  2:1075510/1‑114 (MQ=255)

                            tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg                                                                                                              >  2:780226/1‑114 (MQ=255)

                            tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg                                                                                                              <  1:1075510/114‑1 (MQ=255)

                            tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAg                                                                                                              <  1:780226/114‑1 (MQ=255)

                            tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg                                                                                     >  2:1207419/1‑139 (MQ=255)

                            tctcAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg                                                                                     >  1:1149090/1‑139 (MQ=255)

                                      cgcgCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCAcc                                                                           >  1:905894/1‑139 (MQ=255)

                                       gtgcACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCg                                                                          >  1:911650/3‑139 (MQ=255)

                                       gcgcACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg                                                                                     >  2:504959/1‑128 (MQ=255)

                                       gcgcACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCgg                                                                                     <  1:504959/128‑1 (MQ=255)

                                                         gcgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCg                                                                          <  2:296908/121‑1 (MQ=255)

                                                         gcgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCg                                                                          >  1:296908/1‑121 (MQ=255)

                                                         gcgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTAc                                                        >  1:1211017/1‑139 (MQ=255)

                                                          cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt                                                                        <  2:773218/122‑1 (MQ=255)

                                                          cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt                                                                        <  2:413601/122‑1 (MQ=255)

                                                          cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt                                                                        >  1:954460/1‑122 (MQ=255)

                                                          cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt                                                                        >  1:773218/1‑122 (MQ=255)

                                                          cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt                                                                        <  2:954460/122‑1 (MQ=255)

                                                          cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCt                                                                        >  1:413601/1‑122 (MQ=255)

                                                          cgAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCACTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACg                                                       >  1:270852/1‑139 (MQ=255)

                                                                cACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGt                                                 <  2:911650/139‑1 (MQ=255)

                                                                       gAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGca                                          >  2:1241064/1‑139 (MQ=255)

                                                                       gAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGca                                          <  1:1137922/139‑1 (MQ=255)

                                                                        aaGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGcac                                         <  1:1207419/139‑1 (MQ=255)

                                                                              aCCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCtc                                   >  2:603049/1‑139 (MQ=255)

                                                                               ccGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCtca                                  <  2:92277/139‑1 (MQ=255)

                                                                                         gAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCa                        <  2:1273205/139‑1 (MQ=255)

                                                                                          aaGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAg                       <  2:1188696/139‑1 (MQ=255)

                                                                                           aGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCACTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGt                      <  2:270852/139‑1 (MQ=255)

                                                                                           aGCTCGGGAGAGCGATCAGCAGGCGGAAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCGCCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGt                      <  2:187581/139‑1 (MQ=255)

                                                                                            gttCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTa                     <  2:959281/137‑1 (MQ=255)

                                                                                               cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCAtt                             <  2:1177965/128‑1 (MQ=255)

                                                                                               cGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCAtt                             >  1:1177965/1‑128 (MQ=255)

                                                                                                 ggAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAg                       >  1:1147811/1‑132 (MQ=255)

                                                                                                 ggAGAGCGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAg                       <  2:1147811/132‑1 (MQ=255)

                                                                                                       cGATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGata          >  1:725563/1‑139 (MQ=255)

                                                                                                        gATCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGatat         <  2:1211017/139‑1 (MQ=255)

                                                                                                         aTCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATAtt        >  2:886042/1‑139 (MQ=255)

                                                                                                          tCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCg                                                                          <  2:736082/72‑1 (MQ=255)

                                                                                                          tCAGCAGGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCg                                                                          >  1:736082/1‑72 (MQ=255)

                                                                                                               agGCGGCAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTtat  <  1:603049/139‑1 (MQ=255)

                                                                                                                    gcAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTtat  >  2:326542/1‑134 (MQ=255)

                                                                                                                    gcAAAACGCTGGTAATTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTtat  <  1:326542/134‑1 (MQ=255)

TTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACCCGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGGGAGAGCGATCAGCAGGCGGCAAAACGCTGGTAGTTTTTTGTTAGCCGGATAAGGCACCGCTGCGTCCGGCAATTTCACCGCTCAGTACATACGTTTACGCTGCGTCGATGCACAGCCTCATCATTTTGCAGTATCCTTAAGATATTCCTTAT  >  NC_000913/819617‑819866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A