Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,216,996	Δ2 bp		[ymgC]	[ymgC]

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,216,995	0	A	.	100.0%	179.8 / NA	41	coding (248/249 nt)	ymgC	Blue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (24/17); total (24/17)
*	NC_000913	1,216,996	0	G	.	100.0%	179.8 / NA	41	coding (249/249 nt)	ymgC	Blue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (24/17); total (24/17)

ACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCT  >  NC_000913/1216864‑1217124

||

aCGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaacttgag                                                                                                                            >  2:676077/1‑132 (MQ=255)

  gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                        <  2:616849/139‑1 (MQ=255)

     tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaa                                                                                                                                  <  1:347091/128‑2 (MQ=255)

     tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaa                                                                                                                                  >  2:347091/1‑127 (MQ=255)

     tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAgg                                                                                                                     >  1:758658/1‑139 (MQ=255)

     tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAgg                                                                                                                     >  1:417337/1‑139 (MQ=255)

                     aaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                     <  2:312423/139‑1 (MQ=255)

                                        gATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCAcc                                                                                         >  1:392432/1‑132 (MQ=255)

                                        gATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCAcc                                                                                         <  2:392432/132‑1 (MQ=255)

                                              tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                        >  1:234840/1‑95 (MQ=255)

                                              tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                        <  2:234840/95‑1 (MQ=255)

                                                ttcaatgttatTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGt                                                                          >  1:152039/9‑139 (MQ=255)

                                                              aGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCg                                                            >  1:75576/1‑139 (MQ=255)

                                                                   aTTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                       <  1:676077/139‑1 (MQ=255)

                                                                     tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCa                                                                                                    <  1:613564/92‑1 (MQ=255)

                                                                     tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCa                                                                                                    >  2:613564/1‑92 (MQ=255)

                                                                       aaaTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCttt                                                   <  2:758658/139‑1 (MQ=255)

                                                                          tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtt                                                >  2:179042/1‑139 (MQ=255)

                                                                          tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtt                                                >  1:1885/1‑139 (MQ=255)

                                                                            cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                        <  2:769284/65‑1 (MQ=255)

                                                                            cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                        >  1:769284/1‑65 (MQ=255)

                                                                                 gacgacACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGt                                         >  1:570333/1‑139 (MQ=255)

                                                                                   cTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                       <  2:450737/139‑1 (MQ=255)

                                                                                      cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                     >  2:576643/1‑74 (MQ=255)

                                                                                      cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                     >  2:619225/1‑74 (MQ=255)

                                                                                      cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                     <  1:619225/74‑1 (MQ=255)

                                                                                      cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                     <  1:576643/74‑1 (MQ=255)

                                                                                      cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                       <  1:791729/104‑1 (MQ=255)

                                                                                      cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                       >  2:791729/1‑104 (MQ=255)

                                                                                         tAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGAcc                                       >  2:510021/1‑133 (MQ=255)

                                                                                         tAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCATTCTTATTATGTcc                                       <  1:510021/133‑1 (MQ=255)

                                                                                           aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                               >  1:696274/1‑139 (MQ=255)

                                                                                           aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                               >  1:656523/1‑139 (MQ=255)

                                                                                              gATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                       <  1:173728/96‑1 (MQ=255)

                                                                                              gATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                       >  2:173728/1‑96 (MQ=255)

                                                                                              gATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAgg                            >  1:310899/1‑139 (MQ=255)

                                                                                                       attaATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGaa                   >  1:699886/1‑139 (MQ=255)

                                                                                                             gACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgaga             >  1:87658/1‑139 (MQ=255)

                                                                                                                aGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                 >  1:780889/1‑84 (MQ=255)

                                                                                                                aGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                 <  2:780889/84‑1 (MQ=255)

                                                                                                                aGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGtt          >  2:691305/1‑139 (MQ=255)

                                                                                                                      tCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                       <  2:568659/88‑1 (MQ=255)

                                                                                                                      tCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                       >  1:568659/1‑88 (MQ=255)

                                                                                                                        cGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGctct  <  1:691305/139‑1 (MQ=255)

||

ACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCT  >  NC_000913/1216864‑1217124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A