Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,216,996 Δ2 bp [ymgC] [ymgC]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,216,9950A.100.0% 234.9 / NA 57coding (248/249 nt)ymgCBlue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (26/31);  total (26/31)
*NC_0009131,216,9960G.100.0% 234.9 / NA 57coding (249/249 nt)ymgCBlue light, low temperature and stress induced protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (26/31);  total (26/31)

GCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NC_000913/1216859‑1217125
                                                                                                                                        ||                                                                                                                                 
gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  >  2:745529/1‑137 (MQ=255)
gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  >  2:437771/1‑137 (MQ=255)
gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  <  2:19623/139‑3 (MQ=255)
       gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  2:374297/139‑1 (MQ=255)
       gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  2:209168/139‑1 (MQ=255)
       gCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  2:1125738/139‑1 (MQ=255)
                         aaaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACAt                                                                                                       >  1:1181937/1‑139 (MQ=255)
                          aaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:437771/139‑1 (MQ=255)
                            ccAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:745529/139‑1 (MQ=255)
                                          tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGc                                                                                      >  2:358056/1‑139 (MQ=255)
                                              aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  >  2:438369/1‑91 (MQ=255)
                                              aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTaac                                                                                                                                  <  1:438369/93‑3 (MQ=255)
                                                   tATCTGCAAATATTGCAGTACATTGAAAGGCATAGGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaaa                                                                             >  2:975131/1‑139 (MQ=255)
                                                        gCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:779267/139‑1 (MQ=255)
                                                           aaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                          >  2:611901/1‑134 (MQ=255)
                                                           aaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                          <  1:611901/134‑1 (MQ=255)
                                                               ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:1023593/104‑1 (MQ=255)
                                                               ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    >  2:1023593/1‑104 (MQ=255)
                                                                   aGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      >  1:502094/1‑98 (MQ=255)
                                                                   aGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  2:502094/98‑1 (MQ=255)
                                                                        aTTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:341682/139‑1 (MQ=255)
                                                                          tGAAATGCATACGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATTCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  1:975131/139‑1 (MQ=255)
                                                                          tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:20903/137‑1 (MQ=255)
                                                                          tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        >  2:20903/1‑137 (MQ=255)
                                                                               tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  1:767162/1‑116 (MQ=255)
                                                                               tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  2:767162/116‑1 (MQ=255)
                                                                                 cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACAt                                                                                                       <  1:1202050/83‑1 (MQ=255)
                                                                                 cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACAt                                                                                                       >  2:1202050/1‑83 (MQ=255)
                                                                                 cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaaa                                                                             >  2:59039/1‑109 (MQ=255)
                                                                                 cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaaa                                                                             <  1:59039/109‑1 (MQ=255)
                                                                                        cTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        <  2:1181937/139‑1 (MQ=255)
                                                                                           cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGTGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCa                                     >  2:1415986/1‑139 (MQ=255)
                                                                                               aaCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 <  1:358056/139‑1 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         <  1:974590/50‑1 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTa                                                                                                                         >  2:974590/1‑50 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      >  2:644061/1‑69 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:644061/69‑1 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:264239/99‑1 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  2:264239/1‑99 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      >  1:360833/1‑117 (MQ=255)
                                                                                                aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  2:360833/117‑1 (MQ=255)
                                                                                                             ttaATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                              >  1:646779/1‑112 (MQ=255)
                                                                                                             ttaATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                              <  2:646779/112‑1 (MQ=255)
                                                                                                              taATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:948656/85‑1 (MQ=255)
                                                                                                              taATGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  2:948656/1‑85 (MQ=255)
                                                                                                                aTGACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGTGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                <  1:1415986/139‑1 (MQ=255)
                                                                                                                  gACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGaa                                                                                                              >  2:943314/1‑43 (MQ=255)
                                                                                                                  gACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGaa                                                                                                              <  1:943314/43‑1 (MQ=255)
                                                                                                                  gACAGGGAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgaga              >  1:1067972/1‑139 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTAt                                                                                              <  2:1489647/54‑1 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTAt                                                                                              >  1:1489647/1‑54 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:638117/76‑1 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        >  2:638117/1‑76 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCa                        <  1:680595/124‑1 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCa                        >  2:680595/1‑124 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtct         >  2:758782/1‑139 (MQ=255)
                                                                                                                       ggAATCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtct         >  2:848866/1‑139 (MQ=255)
                                                                                                                         aaTCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTc                                         >  1:277942/1‑105 (MQ=255)
                                                                                                                         aaTCCGTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTc                                         <  2:277942/105‑1 (MQ=255)
                                                                                                                              gTGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc  <  2:1067972/139‑1 (MQ=255)
                                                                                                                               tGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                       >  2:596297/1‑117 (MQ=255)
                                                                                                                               tGCTCTCTT‑‑AACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                       <  1:596297/117‑1 (MQ=255)
                                                                                                                                        ||                                                                                                                                 
GCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGAACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NC_000913/1216859‑1217125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A