Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,474,309 |
G→C |
G30A (GGC→GCC) |
ydbC → |
putative NAD(P)‑binding oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,474,309 | 0 | G | C | 100.0%
| 96.2
/ NA
| 29 | G30A (GGC→GCC) | ydbC | putative NAD(P)‑binding oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (14/15); total (14/15) |
TTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAA > NC_000913/1474173‑1474414
|
tttCTTTACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGccc < 2:408598/139‑1 (MQ=255)
aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGAt > 2:506983/1‑139 (MQ=255)
aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGAt > 1:400830/1‑139 (MQ=255)
aTCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACg > 2:454755/1‑136 (MQ=255)
aTCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACg < 1:454755/136‑1 (MQ=255)
ttattaCAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAAc < 2:223931/139‑1 (MQ=255)
gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGtgctgc > 2:253763/1‑139 (MQ=255)
ggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCg > 1:744770/1‑139 (MQ=255)
cagggTGCGATGATCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTg < 2:245719/139‑1 (MQ=255)
tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg < 1:487256/139‑1 (MQ=255)
tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg < 2:400830/139‑1 (MQ=255)
cGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCt < 1:651241/139‑1 (MQ=255)
aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt < 1:253763/139‑1 (MQ=255)
cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCaa > 2:587128/1‑139 (MQ=255)
aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc > 1:196745/1‑136 (MQ=255)
aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc < 2:196745/136‑1 (MQ=255)
ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCAc > 1:367985/1‑84 (MQ=255)
ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCAc < 2:367985/84‑1 (MQ=255)
aaaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCGACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc < 1:506983/139‑1 (MQ=255)
tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAG‑TTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATg < 2:116718/139‑1 (MQ=255)
ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa > 1:388587/1‑139 (MQ=255)
tGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGAt < 1:542670/49‑1 (MQ=255)
tGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGAt > 2:542670/1‑49 (MQ=255)
tGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCAcc > 2:770931/1‑139 (MQ=255)
ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAtt > 2:390856/1‑89 (MQ=255)
ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAtt < 1:390856/89‑1 (MQ=255)
ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCa < 1:587128/139‑1 (MQ=255)
gcgcGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCAAGTCACCaa > 1:84388/1‑139 (MQ=255)
tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTg < 1:690105/46‑1 (MQ=255)
tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTg > 2:690105/1‑46 (MQ=255)
|
TTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAA > NC_000913/1474173‑1474414
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
