Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,474,309	G→C	G30A (GGC→GCC)	ydbC →	putative NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,474,309	0	G	C	100.0%	167.7 / NA	46	G30A (GGC→GCC)	ydbC	putative NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (25/21); total (25/21)

ATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTT  >  NC_000913/1474184‑1474443

aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGAt                                                                                                                           >  2:10331/1‑139 (MQ=255)

  ttttCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCg                                                                                                                         <  1:313094/139‑1 (MQ=255)

   tttCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGc                                                                                                                        >  2:108648/1‑139 (MQ=255)

                          ggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCg                                                                                                 >  1:525773/1‑139 (MQ=255)

                            cagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTg                                                                                               >  2:53646/1‑139 (MQ=255)

                            cagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTg                                                                                               >  2:84137/1‑139 (MQ=255)

                             agggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGa                                                                                              <  1:947399/139‑1 (MQ=255)

                               ggTGCGATGAGCAGCAATACATTTACTCTGGGTACAAAATCCGTTAACCGTCTTGGTTAAGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCAc                                                                                                                     <  1:665531/114‑1 (MQ=255)

                               ggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCAc                                                                                                                     >  2:665531/1‑114 (MQ=255)

                               ggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAgg                                                                                            >  1:967666/1‑139 (MQ=255)

                               ggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAgg                                                                                            >  1:912718/1‑139 (MQ=255)

                                 tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          >  2:607376/1‑139 (MQ=255)

                                    gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgc                                                                                           >  1:359585/1‑135 (MQ=255)

                                    gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgc                                                                                           <  2:359585/135‑1 (MQ=255)

                                           agcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGt                                                                                                                   <  1:321527/104‑1 (MQ=255)

                                           agcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGt                                                                                                                   >  2:321527/1‑104 (MQ=255)

                                           agcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTgg                                                                                <  2:967666/139‑1 (MQ=255)

                                             caATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGc                                                                              <  1:84137/139‑1 (MQ=255)

                                             caATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGc                                                                              <  2:525773/139‑1 (MQ=255)

                                               aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            <  1:108648/139‑1 (MQ=255)

                                                   aTTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                        <  2:912718/139‑1 (MQ=255)

                                                       aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                                                                    >  2:28133/1‑139 (MQ=255)

                                                        ctctCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCatat                                                                   >  2:995286/1‑139 (MQ=255)

                                                           tcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGt                                                                                                                   >  2:624744/1‑88 (MQ=255)

                                                           tcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGt                                                                                                                   <  1:624744/88‑1 (MQ=255)

                                                             ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  1:101407/111‑1 (MQ=255)

                                                             ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          >  2:101407/1‑111 (MQ=255)

                                                             ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATa                                                              >  2:473953/1‑139 (MQ=255)

                                                                               cGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTc                                            <  1:53646/139‑1 (MQ=255)

                                                                               cGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTc                                            <  1:10331/139‑1 (MQ=255)

                                                                                   ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                        >  2:881078/1‑139 (MQ=255)

                                                                                     ggTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACg                                      <  1:607376/139‑1 (MQ=255)

                                                                                       ttATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  1:152763/85‑1 (MQ=255)

                                                                                       ttATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          >  2:152763/1‑85 (MQ=255)

                                                                                                gATGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAg                           >  2:848541/1‑139 (MQ=255)

                                                                                                 aTGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCAc                                                                                                                     >  2:401298/1‑48 (MQ=255)

                                                                                                 aTGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCAc                                                                                                                     <  1:401298/48‑1 (MQ=255)

                                                                                                 aTGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                        <  2:380255/93‑1 (MQ=255)

                                                                                                 aTGCAACTGGCAGGTCCTGGAGTTTTTGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                        >  1:380255/1‑93 (MQ=255)

                                                                                                                         tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          <  1:696365/51‑1 (MQ=255)

                                                                                                                         tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                          >  2:696365/1‑51 (MQ=255)

                                                                                                                         tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            >  1:62865/1‑65 (MQ=255)

                                                                                                                         tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                            <  2:62865/65‑1 (MQ=255)

                                                                                                                         tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                                                                    >  1:858096/1‑73 (MQ=255)

                                                                                                                         tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                                                                    <  2:858096/73‑1 (MQ=255)

                                                                                                                         tttGCCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCtt  >  1:914907/1‑139 (MQ=255)

ATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTT  >  NC_000913/1474184‑1474443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A