Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,719,058 |
G→A |
P182L (CCG→CTG) |
anmK ← |
anhydro‑N‑acetylmuramic acid kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,719,058 | 0 | G | A | 100.0%
| 101.4
/ NA
| 32 | P182L (CCG→CTG) | anmK | anhydro‑N‑acetylmuramic acid kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/15); total (17/15) |
TTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCA > NC_000913/1718928‑1719184
|
ttGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAG‑CCCCAACCAGCTGCCCAg > 2:595807/1‑139 (MQ=255)
gTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAG‑CCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCa < 1:595807/139‑1 (MQ=255)
tGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCa < 1:397203/139‑1 (MQ=255)
tCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcgtc < 2:197887/139‑1 (MQ=255)
aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc < 2:409015/74‑1 (MQ=255)
aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc > 1:409015/1‑74 (MQ=255)
aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGaa > 2:718699/1‑80 (MQ=255)
aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGaa < 1:718699/80‑1 (MQ=255)
cTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCa < 1:703400/103‑1 (MQ=255)
cTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCa > 2:703400/1‑103 (MQ=255)
ggCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATgg > 2:114544/1‑139 (MQ=255)
ggCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATgg < 1:261271/139‑1 (MQ=255)
catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa > 1:693395/1‑139 (MQ=255)
catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa > 1:476873/1‑139 (MQ=255)
catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa > 1:182445/1‑139 (MQ=255)
catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa < 2:693395/139‑1 (MQ=255)
gTTATCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGTAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACg < 1:554730/128‑1 (MQ=255)
gTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACg > 2:554730/1‑128 (MQ=255)
aCCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAg > 1:571020/1‑45 (MQ=255)
aCCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAg < 2:571020/45‑1 (MQ=255)
cAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCAt > 1:307126/1‑82 (MQ=255)
cAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCAt < 2:307126/82‑1 (MQ=255)
ccGGTATCGTAGCCCCCAACCAGCTGCCCTGGAATGAGCAGt > 2:250856/1‑42 (MQ=255)
ccGGTATCGTAGCCCCCAACCAGCTGCCCTGGAATGAGCAGt < 1:250856/42‑1 (MQ=255)
cGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTg > 2:804812/1‑139 (MQ=255)
ggTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGt < 2:182445/139‑1 (MQ=255)
ggTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGt < 2:476873/139‑1 (MQ=255)
gTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGtc > 1:429509/1‑139 (MQ=255)
gTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGtc > 2:782735/1‑139 (MQ=255)
gTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatga < 2:284167/103‑1 (MQ=255)
gTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatga > 1:284167/1‑103 (MQ=255)
tAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCa > 2:837348/1‑139 (MQ=255)
|
TTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCA > NC_000913/1718928‑1719184
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
