Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,719,058 |
G→A |
P182L (CCG→CTG) |
anmK ← |
anhydro‑N‑acetylmuramic acid kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,719,058 | 0 | G | A | 100.0%
| 72.8
/ NA
| 25 | P182L (CCG→CTG) | anmK | anhydro‑N‑acetylmuramic acid kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/10); total (15/10) |
CAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCA > NC_000913/1718931‑1719156
|
cagcagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGa > 1:179351/1‑139 (MQ=255)
cagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTACCGTAGCCCCCAACCAGCTGCCCAGGAATg < 1:292190/139‑1 (MQ=255)
agTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc > 1:261715/1‑130 (MQ=255)
agTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc < 2:261715/130‑1 (MQ=255)
gaATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGa < 2:136256/139‑1 (MQ=255)
cattACCCCCCCGTGCCCACTCGGAAGCGTTATCGTAAGTTTTACCGGCATGACGCCACATCCAGGCATCCCTCAGCCTGATACCAGGACCGGTATCATAGCCCCCAACCAGCTGCCCAGGAATGACCAGTGACAGAtt > 2:442199/3‑139 (MQ=255)
cTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACTAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGAtt > 1:422665/1‑139 (MQ=255)
ttACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTgg > 1:174628/1‑139 (MQ=255)
cccgcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGa > 1:343663/1‑139 (MQ=255)
gcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGAAGTGACAGATTGGCGATgc > 2:608766/1‑139 (MQ=255)
cccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATgcc > 2:335070/1‑139 (MQ=255)
cgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATgccgc < 2:343663/139‑1 (MQ=255)
aCTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATAtt < 2:174628/139‑1 (MQ=255)
ggTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCgg < 1:335070/139‑1 (MQ=255)
aCCGGCCTGTCGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACTGGTATCGTAGCCCCCAAGCAGCTGCTCAGGAATGAGCAGAGACAGATTGCCGTTGCGGCCAATATTGAGCACCATTCGACGCTGGATTggt > 2:438568/1‑138 (MQ=255)
aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc > 1:919508/1‑74 (MQ=255)
aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc < 2:919508/74‑1 (MQ=255)
cGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCcagca < 1:464602/139‑1 (MQ=255)
aTCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGATAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCat > 1:229174/1‑139 (MQ=255)
cAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatgat < 2:179351/139‑1 (MQ=255)
catccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGaa > 2:2906/1‑139 (MQ=255)
catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa > 2:607104/1‑139 (MQ=255)
catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa > 2:797385/1‑139 (MQ=255)
cccAACCAGCTGCCCAGGAATGAGCAGTGGCAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa < 2:666912/106‑1 (MQ=255)
cccAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa > 1:666912/1‑106 (MQ=255)
|
CAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCA > NC_000913/1718931‑1719156
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
