Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,058	G→A	P182L (CCG→CTG)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,058	0	G	A	100.0%	111.2 / NA	36	P182L (CCG→CTG)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/14); total (22/14)

ATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAA  >  NC_000913/1718925‑1719190

atTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGc                                                                                                                                 >  2:439085/1‑139 (MQ=255)

       agcagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGaa                                                                                                                          >  2:360763/1‑139 (MQ=255)

           gTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAg                                                                                                                      <  1:360763/139‑1 (MQ=255)

                      aCTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGAt                                                                                                           >  2:184263/1‑139 (MQ=255)

                          tACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCTTGACGCCAGATCTAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGc                                                                                                       >  1:464435/1‑139 (MQ=255)

                                          cTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCaa                                                                                            >  1:1101329/1‑134 (MQ=255)

                                          cTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCaa                                                                                            <  2:1101329/134‑1 (MQ=255)

                                           tCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAg                                                                                                                      <  2:878283/107‑1 (MQ=255)

                                           tCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAg                                                                                                                      >  1:878283/1‑107 (MQ=255)

                                                         gTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcgtcga                                                                        <  1:184263/139‑2 (MQ=255)

                                                             ggTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGt                                                                                                                  >  1:361101/1‑93 (MQ=255)

                                                             ggTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGt                                                                                                                  <  2:361101/93‑1 (MQ=255)

                                                                                  aTCCAGGATTCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAAATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCat                                               <  2:811577/139‑1 (MQ=255)

                                                                                       ggCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGAt                                                                                                    <  1:1266532/81‑1 (MQ=255)

                                                                                       ggCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGAt                                                                                                    >  2:1266532/1‑81 (MQ=255)

                                                                                          atccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCa                                                                                             <  1:1189221/85‑1 (MQ=255)

                                                                                          atccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCa                                                                                             >  2:1189221/1‑85 (MQ=255)

                                                                                           tccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGAAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACg                                      >  1:476382/1‑139 (MQ=255)

                                                                                            ccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGc                                     >  2:667342/1‑139 (MQ=255)

                                                                                            ccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGc                                     >  2:1145693/1‑139 (MQ=255)

                                                                                             catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGaa                                                                                                                          <  1:800346/53‑1 (MQ=255)

                                                                                             catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGaa                                                                                                                          >  2:800346/1‑53 (MQ=255)

                                                                                                      gTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGc                                                          >  2:462636/1‑108 (MQ=255)

                                                                                                      gTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGc                                                          <  1:462636/108‑1 (MQ=255)

                                                                                                         aCCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGt                        <  2:37396/139‑1 (MQ=255)

                                                                                                             ggACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTgcgc                    >  2:9914/1‑139 (MQ=255)

                                                                                                             ggACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTAGGGTGAGCCAGCAGCGCCTGATGGAACGCAGGTACCAGCGGTgcgc                    >  1:605894/1‑139 (MQ=255)

                                                                                                                  ggTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAg                                                                                                                      <  1:104866/36‑1 (MQ=255)

                                                                                                                  ggTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAg                                                                                                                      >  2:104866/1‑36 (MQ=255)

                                                                                                                   gTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGtc              >  2:122264/1‑139 (MQ=255)

                                                                                                                       cGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGtcctcc          <  2:464435/139‑1 (MQ=255)

                                                                                                                         tAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCa        >  1:658487/1‑139 (MQ=255)

                                                                                                                           gCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAg      >  2:994657/1‑139 (MQ=255)

                                                                                                                            cccccAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatgatg                                           <  1:826040/101‑1 (MQ=255)

                                                                                                                            cccccAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatgatg                                           >  2:826040/1‑101 (MQ=255)

                                                                                                                              cccAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACG‑AGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCaa  >  1:849046/1‑139 (MQ=255)

ATTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAA  >  NC_000913/1718925‑1719190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A