Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,226,509 |
C→T |
*254* (TAG→TAA) |
yohF ← |
putative oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,226,509 | 0 | C | T | 100.0%
| 85.5
/ NA
| 28 | *254* (TAG→TAA) | yohF | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/13); total (15/13) |
GTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGC > NC_000913/2226376‑2226634
|
gTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTc > 2:417440/1‑139 (MQ=255)
gATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGAt > 1:296832/1‑139 (MQ=255)
ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt < 2:291370/116‑1 (MQ=255)
ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt > 1:291370/1‑116 (MQ=255)
ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc > 1:5802/1‑139 (MQ=255)
tCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCa < 2:441299/139‑1 (MQ=255)
ccATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACt < 2:5802/139‑1 (MQ=255)
tGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc < 1:421102/139‑1 (MQ=255)
tGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACAAAAATATCACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCTCCAACATAAAGCCGCCAGCCACTATCAACGACTGcc < 1:437612/139‑1 (MQ=255)
cTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg > 2:773125/1‑139 (MQ=255)
cTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATaa > 1:235543/1‑89 (MQ=255)
cTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATaa < 2:235543/89‑1 (MQ=255)
ccGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGc > 1:621133/1‑139 (MQ=255)
aCCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac > 2:38742/1‑139 (MQ=255)
tACCCAATGAAATATCCACGCACCTCACTGCGTTAtt < 2:595722/37‑1 (MQ=255)
tACCCAATGAAATATCCACGCACCTCACTGCGTTAtt > 1:595722/1‑37 (MQ=255)
cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATa < 2:828680/64‑1 (MQ=255)
cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATa > 1:828680/1‑64 (MQ=255)
ccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTgg < 1:773125/139‑1 (MQ=255)
ccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTgg < 1:704411/139‑1 (MQ=255)
tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgcc > 2:256498/1‑64 (MQ=255)
tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgcc < 1:256498/64‑1 (MQ=255)
aCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGtt > 1:195421/1‑139 (MQ=255)
gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCa < 2:275396/56‑1 (MQ=255)
gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCa > 1:275396/1‑56 (MQ=255)
gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTgc > 2:837918/1‑139 (MQ=255)
tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCg < 1:738161/130‑1 (MQ=255)
tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCg > 2:738161/1‑130 (MQ=255)
|
GTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGC > NC_000913/2226376‑2226634
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
