Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,226,509	C→T	254 (TAG→TAA)	yohF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,226,509	0	C	T	100.0%	143.5 / NA	46	254 (TAG→TAA)	yohF	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/24); total (22/24)

GGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGC  >  NC_000913/2226398‑2226634

ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAACATATTACCCACTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                    >  1:517637/1‑139 (MQ=255)

ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                           >  1:752465/1‑116 (MQ=255)

ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                           <  2:752465/116‑1 (MQ=255)

ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGtt                                                                                                                    >  2:494558/1‑123 (MQ=255)

ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGtt                                                                                                                    <  1:494558/123‑1 (MQ=255)

ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                    >  1:1347116/1‑139 (MQ=255)

ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                    >  2:642922/1‑139 (MQ=255)

     tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTggg                                                                                                                      <  2:223094/116‑1 (MQ=255)

     tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTggg                                                                                                                      >  1:223094/1‑116 (MQ=255)

       gcgcCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATaa                                                                                             <  2:517637/139‑1 (MQ=255)

              aTCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccgcc                                                                                      <  1:642922/139‑1 (MQ=255)

                 cGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATc                                                                                   <  2:1347116/139‑1 (MQ=255)

                                cATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                 <  1:883233/126‑1 (MQ=255)

                                cATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                 >  2:883233/1‑126 (MQ=255)

                                    ccTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                                <  1:410028/139‑1 (MQ=255)

                                     cTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACt                                                                     <  1:157350/133‑1 (MQ=255)

                                     cTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACt                                                                     >  2:157350/1‑133 (MQ=255)

                                     cTGATACAACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                           >  1:376769/1‑79 (MQ=255)

                                     cTGATACAACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                           <  2:376769/79‑1 (MQ=255)

                                         tACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCggtggt                                                           >  1:171140/1‑139 (MQ=255)

                                               gCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAAtt                                                     >  2:846948/1‑139 (MQ=255)

                                                  tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                           <  1:158358/66‑1 (MQ=255)

                                                  tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                           >  2:158358/1‑66 (MQ=255)

                                                    tgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgtgc                                                >  1:1345339/1‑139 (MQ=255)

                                                        tCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTc                                            <  1:1144522/139‑1 (MQ=255)

                                                         cTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                          <  2:925788/76‑1 (MQ=255)

                                                         cTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                          >  1:925788/1‑76 (MQ=255)

                                                            ttCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGTGCCCTCCGaa                                        <  2:1345339/139‑1 (MQ=255)

                                                             tCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAAc                                       <  2:255956/139‑1 (MQ=255)

                                                                   cGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                                <  1:234962/108‑1 (MQ=255)

                                                                   cGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                                >  2:234962/1‑108 (MQ=255)

                                                                    gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                                >  2:219979/1‑107 (MQ=255)

                                                                    gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                                <  1:219979/107‑1 (MQ=255)

                                                                    gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCca                                <  1:846948/139‑1 (MQ=255)

                                                                               tACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGgtgt                                                         <  1:299238/103‑1 (MQ=255)

                                                                               tACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGgtgt                                                         >  2:299238/1‑103 (MQ=255)

                                                                                 cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                     >  1:205219/1‑57 (MQ=255)

                                                                                 cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                     <  2:205219/57‑1 (MQ=255)

                                                                                    aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCa                <  1:846577/139‑1 (MQ=255)

                                                                                      tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                        <  1:710170/113‑1 (MQ=255)

                                                                                      tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                        >  2:710170/1‑113 (MQ=255)

                                                                                                aCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                  >  1:695836/1‑77 (MQ=255)

                                                                                                aCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                  <  2:695836/77‑1 (MQ=255)

                                                                                                 cGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                        >  2:391995/1‑102 (MQ=255)

                                                                                                 cGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                        <  1:391995/102‑1 (MQ=255)

                                                                                                  gCACCTCACTGCGTTATTCTGGTTTGAACTGGGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCACGCTGGCAATCTCATGCGTTgc  >  1:974853/1‑139 (MQ=255)

GGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGC  >  NC_000913/2226398‑2226634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A