Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,226,509 C→T *254* (TAG→TAA)  yohF ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,226,5090CT100.0% 197.9 / NA 62*254* (TAG→TAA) yohFputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/27);  total (35/27)

GCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGC  >  NC_000913/2226387‑2226644
                                                                                                                          |                                                                                                                                       
gCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACt                                                                                                                         >  1:372452/1‑139 (MQ=255)
     ggATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                                    <  1:1636832/139‑1 (MQ=255)
           ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATGTTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                              >  1:1138772/1‑139 (MQ=255)
           ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                              >  2:972119/1‑139 (MQ=255)
           ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                              >  2:1576649/1‑139 (MQ=255)
                tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                               <  1:351100/133‑1 (MQ=255)
                tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                               >  2:351100/1‑133 (MQ=255)
                tCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACAt                                                                                                         >  1:1128101/1‑139 (MQ=255)
                       gcATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTGATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                                  <  2:1138772/139‑1 (MQ=255)
                              aCATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                           <  2:372452/139‑1 (MQ=255)
                                             tGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACt                                                                               <  2:1644275/136‑1 (MQ=255)
                                             tGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACt                                                                               >  1:1644275/1‑136 (MQ=255)
                                                 tGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGa                                                                                 <  2:438534/130‑1 (MQ=255)
                                                 tGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGa                                                                                 >  1:438534/1‑130 (MQ=255)
                                                  gATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     >  1:817405/1‑109 (MQ=255)
                                                  gATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     <  2:817405/109‑1 (MQ=255)
                                                        acgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                                  <  2:1003264/106‑1 (MQ=255)
                                                        acgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                                  >  1:1003264/1‑106 (MQ=255)
                                                         cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAAt                                                                >  1:945831/1‑139 (MQ=255)
                                                         cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAAt                                                                >  1:533769/1‑139 (MQ=255)
                                                         cgCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAAt                                                                >  1:442780/1‑139 (MQ=255)
                                                             tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACt                                                                                                                         <  1:949680/78‑1 (MQ=255)
                                                             tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACt                                                                                                                         >  2:949680/1‑78 (MQ=255)
                                                             tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgt                                                            <  1:972119/139‑2 (MQ=255)
                                                             tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgc                                                            >  2:1177454/1‑139 (MQ=255)
                                                               tgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                                  <  1:1219037/99‑1 (MQ=255)
                                                               tgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                                  >  2:1219037/1‑99 (MQ=255)
                                                               tgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgcgc                                                          >  2:456191/1‑139 (MQ=255)
                                                                  gTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCt                                                       <  2:533769/139‑1 (MQ=255)
                                                                           tGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACaaa                                              <  2:442780/139‑1 (MQ=255)
                                                                            gCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAg                                             <  2:1128101/139‑1 (MQ=255)
                                                                               gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAATAAAGCca                                          <  1:1177454/139‑1 (MQ=255)
                                                                               gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCca                                          <  2:945831/139‑1 (MQ=255)
                                                                               gACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCca                                          >  1:1516926/1‑139 (MQ=255)
                                                                                aCCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac                                         >  1:103713/1‑139 (MQ=255)
                                                                                aCCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCcac                                         >  2:1146674/1‑139 (MQ=255)
                                                                                          tACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGa                                                                                 >  1:3914/1‑89 (MQ=255)
                                                                                          tACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGa                                                                                 <  2:3914/89‑1 (MQ=255)
                                                                                            cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     >  2:914735/1‑67 (MQ=255)
                                                                                            cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     <  1:914735/67‑1 (MQ=255)
                                                                                            cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTg                             >  1:234648/1‑139 (MQ=255)
                                                                                               aaTGAAATATCCCCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCa                          <  1:1567818/139‑1 (MQ=255)
                                                                                               aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     <  1:465600/64‑1 (MQ=255)
                                                                                               aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     >  2:465600/1‑64 (MQ=255)
                                                                                               aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATc                                                                                      >  1:1545651/1‑79 (MQ=255)
                                                                                               aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATc                                                                                      <  2:1545651/79‑1 (MQ=255)
                                                                                                 tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATaa                                                                                                       <  1:1253842/60‑1 (MQ=255)
                                                                                                 tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATaa                                                                                                       >  2:1253842/1‑60 (MQ=255)
                                                                                                 tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     >  1:1093072/1‑62 (MQ=255)
                                                                                                 tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAg                                                                                                     <  2:1093072/62‑1 (MQ=255)
                                                                                                      tatCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                                  <  1:750411/108‑1 (MQ=255)
                                                                                                      tatCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                                  >  2:750411/1‑108 (MQ=255)
                                                                                                      tatCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCAt                   >  1:1659992/1‑139 (MQ=255)
                                                                                                           aCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGtt              >  2:1139996/1‑139 (MQ=255)
                                                                                                                  tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                                          >  2:686690/1‑72 (MQ=255)
                                                                                                                  tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCg                                                                          <  1:686690/72‑1 (MQ=255)
                                                                                                                  tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                    <  1:244820/110‑1 (MQ=255)
                                                                                                                  tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCAcaccac                                    >  2:244820/1‑110 (MQ=255)
                                                                                                                  tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAg                                 <  2:669522/113‑1 (MQ=255)
                                                                                                                  tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAg                                 >  1:669522/1‑113 (MQ=255)
                                                                                                                  tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCaa       >  1:1728365/1‑139 (MQ=255)
                                                                                                                      tGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAAcg   >  1:1321405/1‑139 (MQ=255)
                                                                                                                       gcgtTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAAcgc  <  2:234648/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                                       
GCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCAAAACGC  >  NC_000913/2226387‑2226644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A