Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A2 F1 I1 R1 | 748 | 32.0 | 1685426 | 92.6% | 1560704 | 103.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,769,486 | G→A | *274* (TAG→TAA) | yfjQ → | CP4‑57 prophage, uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,769,486 | 0 | G | A | 100.0% | 66.8 / NA | 22 | *274* (TAG→TAA) | yfjQ | CP4‑57 prophage, uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/11); total (11/11) | |||||||||||
GGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCT > NC_000913/2769354‑2769596 | gggCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGttt > 1:10673/1‑139 (MQ=255) gTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCAc < 2:110890/139‑1 (MQ=255) gACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGAcaccac < 1:563213/139‑1 (MQ=255) tgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAatat < 1:240158/108‑1 (MQ=255) tgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAatat > 2:240158/1‑108 (MQ=255) tAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGacac > 1:512020/1‑87 (MQ=255) tAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGacac < 2:512020/87‑1 (MQ=255) ccGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCtttt > 1:792642/1‑139 (MQ=255) gcgcTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc < 2:792642/139‑1 (MQ=255) tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCAc > 1:459543/1‑58 (MQ=255) tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCAc < 2:459543/58‑1 (MQ=255) tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGt > 1:691441/1‑83 (MQ=255) tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGt < 2:691441/83‑1 (MQ=255) gATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCAc > 1:330113/1‑51 (MQ=255) gATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCAc < 2:330113/51‑1 (MQ=255) cAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCt > 2:294579/1‑139 (MQ=255) gAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTg < 1:674714/40‑1 (MQ=255) gAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTg > 2:674714/1‑40 (MQ=25) cTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCAt < 1:348861/66‑1 (MQ=255) cTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCAt > 2:348861/1‑66 (MQ=255) cAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGa < 2:61102/38‑1 (MQ=255) cAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGa > 1:61102/1‑38 (MQ=255) | GGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCT > NC_000913/2769354‑2769596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |