Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,769,486	G→A	274 (TAG→TAA)	yfjQ →	CP4‑57 prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,769,486	0	G	A	100.0%	155.2 / NA	49	274 (TAG→TAA)	yfjQ	CP4‑57 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/29); total (20/29)

GGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCT  >  NC_000913/2769355‑2769621

ggCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTc                                                                                                                                  >  2:484134/1‑139 (MQ=255)

        cAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGcc                                                                                                                          <  2:14657/139‑1 (MQ=255)

        cAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGcc                                                                                                                          <  2:417513/139‑1 (MQ=255)

         aGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCa                                                                                                                         <  1:618148/139‑1 (MQ=255)

          gTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCAc                                                                                                                        <  2:321177/139‑1 (MQ=255)

           tGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACg                                                                                                                       >  1:1006244/1‑139 (MQ=255)

           tGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACg                                                                                                                       <  1:951731/139‑1 (MQ=255)

           tGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACg                                                                                                                       <  1:905563/139‑1 (MQ=255)

                    tGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAata                                                                                                              <  2:119191/139‑1 (MQ=255)

                        aaaGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCgg                                                                                                          >  1:422870/1‑139 (MQ=255)

                            gCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGacac                                                                                                      <  2:299592/139‑1 (MQ=255)

                               gACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGAcaccac                                                                                                   >  2:53219/1‑139 (MQ=255)

                                aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGATACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACaccacc                                                                                                  >  2:1045524/1‑139 (MQ=255)

                                aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACgttgt                                                                                                                   <  1:629000/122‑1 (MQ=255)

                                aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACgttgt                                                                                                                   >  2:629000/1‑122 (MQ=255)

                                aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAatat                                                                                                             >  1:4577/1‑128 (MQ=255)

                                aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAatat                                                                                                             <  2:4577/128‑1 (MQ=255)

                                aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACaccacc                                                                                                  >  2:617238/1‑139 (MQ=255)

                                   aaCTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGt                                                                                               <  2:422870/139‑1 (MQ=255)

                                    aCTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGCAACAATCCTCACGCAACTGCAGTAAACGTTACATGTTGCCACGTTATTAATATCGGACACCACCTGTc                                                                                              <  2:47736/139‑1 (MQ=25)

                                     cTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTcc                                                                                             <  2:1006244/139‑1 (MQ=255)

                                         tACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCAt                                                                                         <  2:1047398/139‑1 (MQ=255)

                                         tACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCAt                                                                                         <  2:835918/139‑1 (MQ=255)

                                             cgtgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  2:577168/139‑1 (MQ=255)

                                             cgtgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  1:517552/139‑1 (MQ=255)

                                              gtgccgtgcGTGGCATTGACGGGGATGAGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTa                                                                                    >  2:305970/1‑139 (MQ=255)

                                               tgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGcc                                                                                                                          <  1:276675/100‑1 (MQ=255)

                                               tgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGcc                                                                                                                          >  2:276675/1‑100 (MQ=255)

                                               tgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAt                                                                                   >  1:617064/1‑139 (MQ=255)

                                               tgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAt                                                                                   >  2:934477/1‑139 (MQ=255)

                                                gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtg                                                                                  >  2:414390/1‑139 (MQ=255)

                                                gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtg                                                                                  <  1:19853/139‑1 (MQ=255)

                                                                   gggATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATcc                                                               >  1:1430/1‑139 (MQ=255)

                                                                    ggATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATccc                                                              <  2:714103/139‑1 (MQ=255)

                                                                    ggATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATccc                                                              <  1:414390/139‑1 (MQ=255)

                                                                              cTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAg                                                    >  1:995261/1‑139 (MQ=255)

                                                                                       gcgcTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc                                           <  1:617238/139‑1 (MQ=255)

                                                                                             tgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGtt                                     >  2:157269/1‑139 (MQ=255)

                                                                                                gTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTaa                                             >  1:557606/1‑128 (MQ=255)

                                                                                                gTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTaa                                             <  2:557606/128‑1 (MQ=255)

                                                                                                   aTGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCt                               <  2:578512/139‑1 (MQ=255)

                                                                                                   aTGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCt                               <  2:686681/139‑1 (MQ=255)

                                                                                                                tGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGaa                  <  2:21390/139‑1 (MQ=255)

                                                                                                                tGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGaa                  >  2:1025633/1‑139 (MQ=255)

                                                                                                                       gCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGAcacca                                                                                                    >  1:1036662/1‑50 (MQ=255)

                                                                                                                       gCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGAcacca                                                                                                    <  2:1036662/50‑1 (MQ=255)

                                                                                                                            tGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGt      >  2:37575/1‑139 (MQ=255)

                                                                                                                               aGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTc   <  2:995261/139‑1 (MQ=255)

                                                                                                                                gTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCt  <  1:305970/139‑1 (MQ=255)

GGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCT  >  NC_000913/2769355‑2769621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A