Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,422,793 |
C→T |
A203V (GCC→GTC) |
ulaD → |
3‑keto‑L‑gulonate 6‑phosphate decarboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,422,793 | 0 | C | T | 100.0%
| 85.6
/ NA
| 28 | A203V (GCC→GTC) | ulaD | 3‑keto‑L‑gulonate 6‑phosphate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/13); total (15/13) |
CAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAA > NC_000913/4422677‑4422923
|
cAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCg > 1:200621/1‑119 (MQ=255)
aatgtcaccgtcaccGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAGACGTTCCa < 2:506304/136‑1 (MQ=255)
aaaGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc < 2:73410/137‑1 (MQ=255)
aaaGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc > 1:73410/1‑137 (MQ=255)
ccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa < 1:270179/132‑1 (MQ=255)
ccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa > 2:270179/1‑132 (MQ=255)
tGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc < 1:362818/115‑1 (MQ=255)
tGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc > 2:362818/1‑115 (MQ=255)
ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg > 1:394580/1‑131 (MQ=255)
ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGGTCCATCGCTGAACTGTGGGGCTAAg < 2:394580/131‑1 (MQ=255)
aaGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGatat < 2:226082/139‑1 (MQ=255)
ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAATCGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg > 2:600392/1‑139 (MQ=255)
ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg > 2:194868/1‑139 (MQ=255)
ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg > 2:660113/1‑139 (MQ=255)
tCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc < 1:660113/139‑1 (MQ=255)
gATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATc < 1:194868/139‑1 (MQ=255)
tCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt < 1:600392/139‑1 (MQ=255)
ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTc > 1:402221/1‑51 (MQ=255)
ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTc < 2:402221/51‑1 (MQ=255)
ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccg > 2:318375/1‑139 (MQ=255)
aTGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct > 2:679893/1‑139 (MQ=255)
gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCggg > 2:266355/1‑126 (MQ=255)
gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCggg < 1:266355/126‑1 (MQ=255)
gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg > 1:592618/1‑139 (MQ=255)
cGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc > 2:825262/1‑78 (MQ=255)
cGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc < 1:825262/78‑1 (MQ=255)
cGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGaa > 1:337781/1‑139 (MQ=255)
ggTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCaa < 2:337781/139‑1 (MQ=255)
|
CAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAA > NC_000913/4422677‑4422923
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
