Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 770 68.5 3470224 91.7% 3182195 103.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,422,793 C→T A203V (GCC→GTC)  ulaD → 3‑keto‑L‑gulonate 6‑phosphate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,422,7930CT98.6% 224.9 / NA 70A203V (GCC→GTC) ulaD3‑keto‑L‑gulonate 6‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (36/33);  minor base G (0/1);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAA  >  NC_000913/4422662‑4422923
                                                                                                                                   |                                                                                                                                  
ttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGt                                                                                                                             >  2:1266787/1‑139 (MQ=255)
     atatGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGt                                                                                                                             <  1:301335/134‑1 (MQ=255)
     atatGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGt                                                                                                                             >  2:301335/1‑134 (MQ=255)
             ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGtt                                                                                                                >  2:1128390/1‑139 (MQ=255)
              tCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                               <  1:1647875/139‑1 (MQ=255)
                     caccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCt                                                                                                        >  1:82659/1‑139 (MQ=255)
                      accgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTg                                                                                                       <  1:467744/139‑1 (MQ=255)
                          tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACt                                                                                                   >  1:1574440/1‑139 (MQ=255)
                           caccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                  <  1:1629819/139‑1 (MQ=255)
                                   gCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTa                                                                                          <  1:1595075/139‑1 (MQ=255)
                                      tGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                       >  1:1010680/1‑139 (MQ=255)
                                        gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAg                                                                                     >  1:761637/1‑139 (MQ=255)
                                        gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAg                                                                                     >  1:233289/1‑139 (MQ=255)
                                        gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAg                                                                                     >  1:1605160/1‑139 (MQ=255)
                                           cTGGAAGATCTCCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCgg                                                                                  <  1:1128390/139‑1 (MQ=255)
                                             ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGat                                                                                >  2:1586668/1‑139 (MQ=255)
                                               aaGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGatat                                                                              <  2:761637/139‑1 (MQ=255)
                                                         gCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCcaaa                                                                    <  2:1605160/139‑1 (MQ=255)
                                                            gTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                        <  2:1607198/132‑1 (MQ=255)
                                                            gTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                        >  1:1607198/1‑132 (MQ=255)
                                                                    gTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCt                                                         <  1:1266787/139‑1 (MQ=255)
                                                                       ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                        >  2:1133569/1‑105 (MQ=255)
                                                                       ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                        <  1:1133569/105‑1 (MQ=255)
                                                                       ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg                                                      >  1:979659/1‑139 (MQ=255)
                                                                       ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg                                                      >  1:1338698/1‑139 (MQ=255)
                                                                            aTTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCAt                                                   <  2:1508782/137‑1 (MQ=255)
                                                                            aTTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCAt                                                   >  1:1508782/1‑137 (MQ=255)
                                                                            aTTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCGCCGGTGGAAGGCGCACGTCAGTTCAAACGTTCCGTCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTAACCAAACAAATCCCGCTTGGCAttt                                                 <  2:233289/139‑3 (MQ=255)
                                                                                 cGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaa                                            >  2:590689/1‑139 (MQ=255)
                                                                                  gTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAg                                                                                                                          >  2:610918/1‑60 (MQ=255)
                                                                                  gTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAg                                                                                                                          <  1:610918/60‑1 (MQ=255)
                                                                                  gTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                       >  2:1430233/1‑95 (MQ=255)
                                                                                  gTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                       <  1:1430233/95‑1 (MQ=255)
                                                                                       tATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAt                                                               >  2:637355/1‑114 (MQ=255)
                                                                                       tATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAt                                                               <  1:637355/114‑1 (MQ=255)
                                                                                       tATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAgcg                                      >  1:1299367/1‑139 (MQ=255)
                                                                                        aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAgcgc                                     >  2:576060/1‑139 (MQ=255)
                                                                                              ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCg                                                                                                                                  <  2:1101218/40‑1 (MQ=255)
                                                                                              ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                             >  1:1080234/1‑61 (MQ=255)
                                                                                              ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                             <  2:1080234/61‑1 (MQ=255)
                                                                                              ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                     >  1:1438210/1‑117 (MQ=255)
                                                                                              ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                     <  2:1438210/117‑1 (MQ=255)
                                                                                               gCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                     <  1:1568474/116‑1 (MQ=255)
                                                                                               gCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                     >  2:1568474/1‑116 (MQ=255)
                                                                                                   tagtaTCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                               <  1:154839/54‑1 (MQ=255)
                                                                                                   tagtaTCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                               >  2:154839/1‑54 (MQ=255)
                                                                                                         ccGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                             <  1:1488067/50‑1 (MQ=255)
                                                                                                         ccGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                             >  2:1488067/1‑50 (MQ=255)
                                                                                                         ccGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                  >  2:110992/1‑61 (MQ=255)
                                                                                                         ccGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                  <  1:110992/61‑1 (MQ=255)
                                                                                                              aTGTCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgcc                             >  2:648846/1‑125 (MQ=255)
                                                                                                              aTGTCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgcc                             <  1:648846/125‑1 (MQ=255)
                                                                                                              aTGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaaaaa                                         >  2:1469010/1‑113 (MQ=255)
                                                                                                              aTGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaaaaa                                         <  1:1469010/113‑1 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCATTTCAAACGTTCCATCGCTGAACTGTTGGGCTAAGGAGCGGATATGTTGTc                                                                        <  2:593459/80‑1 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtgt                                                                                                 >  1:1715361/1‑55 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtgt                                                                                                 <  2:1715361/55‑1 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                        >  1:593459/1‑80 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                     >  2:874854/1‑99 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                     <  1:874854/99‑1 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg             <  1:1522971/139‑1 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg             >  1:1281109/1‑139 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg             <  2:1574440/139‑1 (MQ=255)
                                                                                                                gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCAAAACAAACCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg             >  1:113299/1‑139 (MQ=255)
                                                                                                                    cTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACg         >  1:549681/1‑139 (MQ=255)
                                                                                                                      tctcCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGcc       <  2:824395/139‑1 (MQ=255)
                                                                                                                          cGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCa   <  2:82659/139‑1 (MQ=255)
                                                                                                                           ggTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCaa  <  2:1299367/139‑1 (MQ=255)
                                                                                                                               gAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGcc       >  1:728582/1‑130 (MQ=255)
                                                                                                                               gAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGcc       <  2:728582/130‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                  
TTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAA  >  NC_000913/4422662‑4422923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A