Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
369,076 |
A→G |
K156E (AAA→GAA) |
mhpA → |
3‑(3‑hydroxyphenyl)propionate hydroxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 369,076 | 0 | A | G | 100.0%
| 107.1
/ NA
| 31 | K156E (AAA→GAA) | mhpA | 3‑(3‑hydroxyphenyl)propionate hydroxylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/12); total (19/12) |
AACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGA > NC_000913/368944‑369202
|
aaCGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACgg > 1:543520/1‑139 (MQ=255)
aTTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGc > 1:507897/1‑139 (MQ=255)
ccGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCggg > 1:696107/1‑139 (MQ=255)
ggTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATa > 2:866571/1‑139 (MQ=255)
gAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTgg < 2:309364/139‑1 (MQ=255)
aGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTa > 1:215531/1‑139 (MQ=255)
aGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTa > 2:22524/1‑139 (MQ=255)
ggTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGcc > 2:177441/1‑139 (MQ=255)
gtgtCGCGTTTTACGAATGTGCGCTGATTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGAGCAGCGGGAAATAGACAAAGCCCAGTGGCTGGTAGCCt > 2:479945/1‑139 (MQ=255)
gtCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCtgt < 2:543520/139‑1 (MQ=255)
tCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCtgtg > 1:177443/1‑139 (MQ=255)
ttttCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGt > 1:21410/1‑139 (MQ=255)
ccGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAg < 1:866571/139‑1 (MQ=255)
cGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGc < 1:177441/139‑1 (MQ=255)
cgcTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTg > 1:92203/1‑139 (MQ=255)
cgcTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTg > 1:572677/1‑139 (MQ=255)
gctTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCg > 1:854167/1‑139 (MQ=255)
tttCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCAc < 2:21410/139‑1 (MQ=255)
cgcgAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGa < 1:459701/62‑1 (MQ=255)
cgcgAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGa > 2:459701/1‑62 (MQ=255)
cTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCa < 1:316656/76‑1 (MQ=255)
cTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCa > 2:316656/1‑76 (MQ=255)
gAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGc > 2:292369/1‑107 (MQ=255)
gAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGc < 1:292369/107‑1 (MQ=255)
ttCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAg > 1:969853/1‑139 (MQ=255)
aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGt < 2:968708/41‑1 (MQ=255)
aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGt > 1:968708/1‑41 (MQ=255)
aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGa < 2:507897/139‑1 (MQ=255)
aCCTTGCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGa < 2:969853/139‑1 (MQ=255)
gCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAg > 2:813196/1‑46 (MQ=255)
gCACCTGGAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAg < 1:813196/46‑1 (MQ=255)
|
AACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGCACTCTGAATGTGCCGTTTGAAGGTAAAACTGCGCCAAATCAGTGGA > NC_000913/368944‑369202
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
