Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 765 46.1 2269152 93.8% 2128464 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 941,832 A→G T292A (ACA→GCA)  dmsA → dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,8320AG100.0% 202.4 / NA 56T292A (ACA→GCA) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/28);  total (28/28)

GTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGAAAACCCTGCCAGCCAGTGCGCCGAAAAATGGC  >  NC_000913/941707‑941963
                                                                                                                             |                                                                                                                                   
gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACt                                                                                                                        >  2:1079694/1‑139 (MQ=255)
 tGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGCTCATCATAGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCAGTg                                                                                                                       <  2:126729/139‑1 (MQ=255)
        aCTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTaa                                                                                                                  >  2:357177/1‑137 (MQ=255)
        aCTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTaa                                                                                                                  <  1:357177/137‑1 (MQ=255)
        aCTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACg                                                                                                                >  1:924746/1‑139 (MQ=255)
          ttACTATCTTGAACAGGCACGCCAGAAATCCAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGt                                                                                                              <  1:583198/139‑1 (MQ=255)
               aTCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGc                                                                                                         >  1:338498/1‑139 (MQ=255)
                tCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATATCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCg                                                                                                        <  2:687425/139‑1 (MQ=255)
                tCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCg                                                                                                        <  2:431820/139‑1 (MQ=255)
                 cTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGt                                                                                                       <  1:596431/139‑1 (MQ=255)
                       cAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCa                                                                                                 <  1:1030662/139‑1 (MQ=255)
                          gCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCg                                                                                                                            >  2:805929/1‑109 (MQ=255)
                          gCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCg                                                                                                                            <  1:805929/109‑1 (MQ=255)
                          gCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCa                                                                                                                          <  2:500876/111‑1 (MQ=255)
                          gCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCa                                                                                                                          >  1:500876/1‑111 (MQ=255)
                          gCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGt                                                                                                              >  1:1100676/1‑123 (MQ=255)
                          gCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGt                                                                                                              <  2:1100676/123‑1 (MQ=255)
                          gCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGa                                                                                              >  2:855103/1‑139 (MQ=255)
                                   aaaTCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGaaa                                                                                     <  2:924746/139‑1 (MQ=255)
                                         aaTGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCtgg                                                                               >  2:468014/1‑139 (MQ=255)
                                              ccGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGAt                                                                          <  1:468014/139‑1 (MQ=255)
                                               cGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATc                                                                         >  2:392325/1‑139 (MQ=255)
                                                 cATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAg                                                                       >  2:634020/1‑139 (MQ=255)
                                                 cATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAg                                                                       >  1:310308/1‑139 (MQ=255)
                                                      tcatcatcGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCAtt                                                                  <  1:634020/139‑1 (MQ=255)
                                                          catcGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCg                                                                                                        >  1:75118/1‑97 (MQ=255)
                                                          catcGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCg                                                                                                        <  2:75118/97‑1 (MQ=255)
                                                            tcGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGaa                                                                                      <  1:563570/113‑1 (MQ=255)
                                                            tcGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGaa                                                                                      >  2:563570/1‑113 (MQ=255)
                                                             cGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGAt                                                           <  1:1079694/139‑1 (MQ=255)
                                                              gATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATa                                                          >  1:245624/1‑139 (MQ=255)
                                                               aTCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCa                                                                                                                          >  1:1079501/1‑74 (MQ=255)
                                                               aTCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCa                                                                                                                          <  2:1079501/74‑1 (MQ=255)
                                                               aTCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGAt                                                                                             >  2:3602/1‑103 (MQ=255)
                                                               aTCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGAt                                                                                             <  1:3602/103‑1 (MQ=255)
                                                               aTCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATaa                                                         >  1:522895/1‑139 (MQ=255)
                                                               aTCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATaa                                                         >  2:189634/1‑139 (MQ=255)
                                                                tCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGt                                                                                                              <  1:797831/85‑1 (MQ=255)
                                                                tCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGt                                                                                                              >  2:797831/1‑85 (MQ=255)
                                                                  cgcgCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAata                                                      <  1:392325/139‑1 (MQ=255)
                                                                    cgcTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATg                                                                                                                               >  2:222626/1‑64 (MQ=255)
                                                                    cgcTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATg                                                                                                                               <  1:222626/64‑1 (MQ=255)
                                                                              acacCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACt                                                                                         >  1:480465/1‑92 (MQ=255)
                                                                              acacCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACt                                                                                         <  2:480465/92‑1 (MQ=255)
                                                                              acacCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTa                                          >  1:953663/1‑139 (MQ=255)
                                                                                 ccGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCa                                                                                                                          <  2:1107937/56‑1 (MQ=255)
                                                                                 ccGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCa                                                                                                                          >  1:1107937/1‑56 (MQ=255)
                                                                                     tGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATgag                                   <  1:189634/139‑1 (MQ=255)
                                                                                             gcgAAGATGAGTGGATCCCTATTCGTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGAAAACCCt                           <  1:855103/139‑1 (MQ=255)
                                                                                                                     gTCCGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGAAAACCCTGCCAGCCAGTGCGCCGAAAAATgg   >  2:560923/1‑139 (MQ=255)
                                                                                                                       ccGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGc                                            <  2:301308/96‑1 (MQ=255)
                                                                                                                       ccGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGc                                            >  1:301308/1‑96 (MQ=255)
                                                                                                                       ccGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGaaaa                               <  2:401441/109‑1 (MQ=255)
                                                                                                                       ccGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGaaaa                               >  1:401441/1‑109 (MQ=255)
                                                                                                                       ccGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGAAAACCCTGCCAGCCAGTGCGCCGAAAAATGGc  <  1:958268/138‑1 (MQ=255)
                                                                                                                       ccGGGAGCAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGAAAACCCTGCCAGCCAGTGCGCCGAAAAATGGc  >  2:958268/1‑138 (MQ=255)
                                                                                                                             |                                                                                                                                   
GTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTTGGCTACGATGAGAAAACCCTGCCAGCCAGTGCGCCGAAAAATGGC  >  NC_000913/941707‑941963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A