Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,693	C→T	P32L (CCG→CTG)	ycfJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,693	0	C	T	100.0%	155.8 / NA	48	P32L (CCG→CTG)	ycfJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/21); total (27/21)

CGCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGT  >  NC_000913/1167557‑1167818

cGCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACCGGGCCTGc                                                                                                                             >  2:964196/1‑139 (MQ=255)

 gCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTGCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCa                                                                                                                            <  2:144708/139‑1 (MQ=255)

      cATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACg                                                                                                                       <  1:391159/139‑1 (MQ=255)

        tGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                     <  2:101826/139‑1 (MQ=255)

           ttGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAg                                                                                                                  >  2:1037861/1‑139 (MQ=255)

            tGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                 >  1:1078268/1‑139 (MQ=255)

             ggCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgt                                                                                                                >  2:173894/1‑139 (MQ=255)

                aTAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgttgt                                                                                                             >  1:888705/1‑139 (MQ=255)

                    cTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCt                                                                                                         >  2:748004/1‑139 (MQ=255)

                       cAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                           <  2:970435/118‑1 (MQ=255)

                       cAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                           >  1:970435/1‑118 (MQ=255)

                        aaaGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACCGGGCCTGCAATACGCTCAGGTTGTTTCTGCaa                                                                                                     <  1:964196/139‑1 (MQ=255)

                          agaagGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   <  1:173894/139‑1 (MQ=255)

                              ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                     >  1:671443/1‑117 (MQ=255)

                              ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGCATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                     <  2:671443/117‑1 (MQ=255)

                               gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                              >  1:133935/1‑139 (MQ=255)

                                        ccGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     <  2:713288/139‑1 (MQ=255)

                                               tAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTaaa                                                                              >  1:29754/1‑139 (MQ=255)

                                                        gTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGt                                                                     >  1:970043/1‑139 (MQ=255)

                                                          tGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     >  1:452784/1‑121 (MQ=255)

                                                          tGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     <  2:452784/121‑1 (MQ=255)

                                                            gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAgg                                                                 >  2:440953/1‑139 (MQ=255)

                                                            gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACACCAATCAAGGAAACGGTTAAAACACCGCGTCAgg                                                                 >  1:728397/1‑139 (MQ=255)

                                                             cGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGa                                                                >  1:285100/1‑139 (MQ=255)

                                                                  aTCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTc                                                           >  2:314088/1‑139 (MQ=255)

                                                                   tCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCg                                                          >  2:389814/1‑139 (MQ=255)

                                                                           ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAccc                                                                                                  <  1:1090251/91‑1 (MQ=255)

                                                                           ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAccc                                                                                                  >  2:1090251/1‑91 (MQ=255)

                                                                           ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTca                                                  <  1:748004/139‑1 (MQ=255)

                                                                             tgtCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                >  1:515504/1‑139 (MQ=255)

                                                                             tgtCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                >  2:1023508/1‑139 (MQ=255)

                                                                                 gCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     <  2:420532/98‑1 (MQ=255)

                                                                                 gCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAAc                                                                                     >  1:420532/1‑98 (MQ=255)

                                                                                      tGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCAt                                       >  2:135127/1‑139 (MQ=255)

                                                                                             ggCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCCATCAAGGaa                                                                                       <  1:761533/84‑1 (MQ=255)

                                                                                             ggCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGaa                                                                                       >  2:761533/1‑84 (MQ=255)

                                                                                             ggCGTAGCGGCAGTGGCCAGGCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGAc                                <  1:389814/139‑1 (MQ=255)

                                                                                               cGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATa                                                                                                                         <  2:516145/48‑1 (MQ=255)

                                                                                               cGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATa                                                                                                                         >  1:516145/1‑48 (MQ=255)

                                                                                               cGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCg                              >  2:838182/1‑139 (MQ=255)

                                                                                                  aGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   <  2:175827/67‑1 (MQ=255)

                                                                                                  aGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   >  1:175827/1‑67 (MQ=255)

                                                                                                           ggCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaa                  <  2:888705/139‑1 (MQ=255)

                                                                                                           ggCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaa                  <  2:970043/139‑1 (MQ=255)

                                                                                                                gTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGCAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCg             <  2:728397/139‑1 (MQ=255)

                                                                                                                           tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   >  1:488410/1‑42 (MQ=255)

                                                                                                                           tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                   <  2:488410/42‑1 (MQ=255)

                                                                                                                           tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGt  <  1:1023508/139‑1 (MQ=255)

CGCTTGCATGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGT  >  NC_000913/1167557‑1167818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A