Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,693	C→T	P32L (CCG→CTG)	ycfJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,693	0	C	T	100.0%	211.5 / NA	65	P32L (CCG→CTG)	ycfJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/32); total (33/32)

TGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCG  >  NC_000913/1167565‑1167827

tGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              <  2:179994/139‑1 (MQ=255)

   ttGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              >  1:463460/1‑136 (MQ=255)

   ttGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              <  2:463460/136‑1 (MQ=255)

                    aagGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcccc                                                                                                          <  1:731879/139‑1 (MQ=255)

                      ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                                    >  1:163597/1‑111 (MQ=255)

                      ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                                    <  2:163597/111‑1 (MQ=255)

                       gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                                       >  2:1540753/1‑139 (MQ=255)

                       gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                                       >  2:1997305/1‑139 (MQ=255)

                       gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                                       >  2:1746840/1‑139 (MQ=255)

                          gtgtTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          >  1:234795/1‑117 (MQ=255)

                          gtgtTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          <  2:234795/117‑1 (MQ=255)

                              tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              >  1:1833194/1‑109 (MQ=255)

                              tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              <  2:1833194/109‑1 (MQ=255)

                              tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGaa                                                                                                >  2:251306/1‑139 (MQ=255)

                              tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGaa                                                                                                >  2:1612690/1‑139 (MQ=255)

                                          aTCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGCACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAaca                                                                                    >  2:1283094/1‑139 (MQ=255)

                                          aTCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCa                                                                                                               <  1:170104/112‑1 (MQ=255)

                                          aTCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCa                                                                                                               >  2:170104/1‑112 (MQ=255)

                                           tCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:1746840/139‑1 (MQ=255)

                                           tCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:251306/139‑1 (MQ=255)

                                           tCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:474738/139‑1 (MQ=255)

                                               tGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACcgcg                                                                               >  1:2054953/1‑139 (MQ=255)

                                               tGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACcgcg                                                                               >  1:924593/1‑139 (MQ=255)

                                                    gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgttgt                                                                                                                      <  1:306610/95‑1 (MQ=255)

                                                    gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgttgt                                                                                                                      >  2:306610/1‑95 (MQ=255)

                                                         tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                            <  1:1714397/100‑1 (MQ=255)

                                                         tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                            >  2:1714397/1‑100 (MQ=255)

                                                         tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   >  2:893336/1‑125 (MQ=255)

                                                         tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:893336/125‑1 (MQ=255)

                                                           tCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcccc                                                                                                          <  1:1251703/100‑1 (MQ=255)

                                                           tCGGGATTGGTGTCACAGCTGCGCTGGGCGTAGCGGCAGTAGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAccct                                                                                                          >  2:1251703/1‑99 (MQ=255)

                                                               gATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAAc                                                               <  1:1997305/139‑1 (MQ=255)

                                                                 ttGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGt                                                             <  2:924593/139‑1 (MQ=255)

                                                                 ttGGTGTCGCAGCTGCGCTGCGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGt                                                             <  1:595852/139‑1 (MQ=255)

                                                                  tGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTc                                                            >  1:1543775/1‑139 (MQ=255)

                                                                   ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTg                                                                                                                 >  2:945224/1‑85 (MQ=255)

                                                                   ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTg                                                                                                                 <  1:945224/85‑1 (MQ=255)

                                                                   ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           <  2:775272/123‑1 (MQ=255)

                                                                   ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           >  1:775272/1‑123 (MQ=255)

                                                                   ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTca                                                           >  2:1920063/1‑139 (MQ=255)

                                                                   ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTca                                                           >  2:1855056/1‑139 (MQ=255)

                                                                        cGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          <  2:616176/71‑1 (MQ=255)

                                                                        cGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          >  1:616176/1‑71 (MQ=255)

                                                                          cAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGAc                                                    >  2:1602836/1‑139 (MQ=255)

                                                                           aGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGAcc                                                   <  2:1118402/139‑1 (MQ=255)

                                                                               gcgcTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           >  2:1258194/1‑111 (MQ=255)

                                                                               gcgcTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           <  1:1258194/111‑1 (MQ=255)

                                                                                  cTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCAtcgtc                                            <  1:1283094/139‑1 (MQ=255)

                                                                                           gCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGc                                   <  1:1920063/139‑1 (MQ=255)

                                                                                                   ggCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaa                           <  1:1612690/139‑1 (MQ=255)

                                                                                                    gCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaaa                          >  2:1141013/1‑139 (MQ=255)

                                                                                                         tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                         <  1:1153336/103‑1 (MQ=255)

                                                                                                         tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                         >  2:1153336/1‑103 (MQ=255)

                                                                                                         tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCa                                  <  1:62166/126‑1 (MQ=255)

                                                                                                         tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCa                                  >  2:62166/1‑126 (MQ=255)

                                                                                                                   tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGc                                                                  <  2:1927709/84‑1 (MQ=255)

                                                                                                                   tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGc                                                                  >  1:1927709/1‑84 (MQ=255)

                                                                                                                   tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCa                                                                 >  1:39394/1‑85 (MQ=255)

                                                                                                                   tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCa                                                                 <  2:39394/85‑1 (MQ=255)

                                                                                                                   tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGt           <  1:1540753/139‑1 (MQ=255)

                                                                                                                       aaCGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGt                                                       <  2:325778/91‑1 (MQ=255)

                                                                                                                       aaCGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGt                                                       >  1:325778/1‑91 (MQ=255)

                                                                                                                          ggggCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCt    <  2:1029636/139‑1 (MQ=255)

                                                                                                                            ggCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCg  >  2:1498762/1‑139 (MQ=255)

                                                                                                                            ggCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGAGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCg  >  1:31188/1‑139 (MQ=255)

TGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCG  >  NC_000913/1167565‑1167827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A