Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,571,332 |
G→T |
I238I (ATC→ATA) |
gadB ← |
glutamate decarboxylase B, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,571,332 | 0 | G | T | 100.0%
| 52.5
/ NA
| 23 | I238I (ATC→ATA) | gadB | glutamate decarboxylase B, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/10); total (13/10) |
TGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTT > NC_000913/1571227‑1571459
|
tGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATcc > 1:545378/1‑139 (MQ=17)
aGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAgc > 2:440807/1‑139 (MQ=17)
tGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCt > 1:929297/1‑120 (MQ=17)
tGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCt < 2:929297/120‑1 (MQ=18)
aTCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATGCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGt > 1:690841/1‑139 (MQ=16)
tttCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGc > 2:866193/1‑139 (MQ=17)
cgGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTggg < 2:690841/139‑1 (MQ=18)
ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGa > 1:486328/1‑139 (MQ=18)
gTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTa < 1:440807/139‑1 (MQ=18)
tCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAc < 1:866193/139‑1 (MQ=17)
atatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTa < 1:176674/129‑1 (MQ=18)
atatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTa > 2:176674/1‑129 (MQ=18)
tatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGTGCATCGTGCAGCGGTTGTgg < 2:669131/115‑1 (MQ=16)
tatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGTGCATCGTGCAGCGGTTGTgg > 1:669131/1‑115 (MQ=12)
gacgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGaaa > 2:428424/1‑139 (MQ=21)
gCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGTTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGcacca < 2:746997/139‑1 (MQ=21)
ccAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGcaccac > 1:651335/1‑139 (MQ=21)
cAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTACCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGTCGAAAGTCGGCACCACg < 2:651335/139‑1 (MQ=255)
ggAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGc < 2:106639/131‑1 (MQ=25)
ggAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGc > 1:106639/1‑131 (MQ=37)
gCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATg < 2:545378/139‑1 (MQ=37)
ccACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATgg > 1:417588/1‑139 (MQ=21)
aTGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCtt > 1:763382/1‑139 (MQ=21)
|
TGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTT > NC_000913/1571227‑1571459
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
