Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,571,332 |
G→T |
I238I (ATC→ATA) |
gadB ← |
glutamate decarboxylase B, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,571,332 | 0 | G | T | 100.0%
| 46.9
/ NA
| 19 | I238I (ATC→ATA) | gadB | glutamate decarboxylase B, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (9/10); total (9/10) |
CTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGG > NC_000913/1571226‑1571441
|
cTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATc > 2:877132/1‑139 (MQ=17)
taGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAg > 2:557370/2‑139 (MQ=18)
cacaCGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGt > 1:699834/1‑139 (MQ=18)
cgGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTggg < 1:1260578/139‑1 (MQ=17)
ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGa > 1:1201246/1‑139 (MQ=17)
cAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAAc < 2:1201246/139‑1 (MQ=18)
cAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAAc < 1:557370/139‑1 (MQ=17)
ggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATa > 1:1162964/1‑117 (MQ=17)
ggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATa < 2:1162964/117‑1 (MQ=18)
gTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGcac < 2:1214127/139‑1 (MQ=37)
gTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGcac < 2:699834/139‑1 (MQ=37)
gCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATg < 1:877132/139‑1 (MQ=21)
ccACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCt < 1:325376/52‑1 (MQ=255)
ccACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCt > 2:325376/1‑52 (MQ=255)
ccACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCa > 2:1112575/1‑69 (MQ=255)
ccACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCa < 1:1112575/69‑1 (MQ=255)
ccACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATgg > 2:116925/1‑139 (MQ=37)
cAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAc > 2:201976/1‑90 (MQ=25)
cAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAc < 1:201976/90‑1 (MQ=25)
|
CTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGG > NC_000913/1571226‑1571441
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
