Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,834,111	G→A	237 (TAG→TAA)	ydjX →	TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,834,111	0	G	A	100.0%	127.9 / NA	40	237 (TAG→TAA)	ydjX	TVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/20); total (20/20)

CAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTTCGAA  >  NC_000913/1833983‑1834246

cAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               <  1:272595/139‑1 (MQ=255)

cAACTCAGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               <  1:664707/139‑1 (MQ=255)

   ctctGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCaa                                                                                                                            >  1:938768/1‑139 (MQ=255)

      tgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               <  2:45419/133‑1 (MQ=255)

      tgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               >  1:45419/1‑133 (MQ=255)

                gCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAgtgt                                                                                                               >  1:976696/1‑139 (MQ=255)

                 ccTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACg                                                                                                                                     >  1:388724/1‑116 (MQ=255)

                 ccTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACg                                                                                                                                     <  2:388724/116‑1 (MQ=255)

                  cTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTGGCAACATTATTCAGTGCCa                                                                                                             >  2:481264/1‑139 (MQ=255)

                                     tCCAGCTCGCAAAACTCTACGCCCGACACAAACCTGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                          >  1:951370/1‑139 (MQ=255)

                                     tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                          >  1:929946/1‑139 (MQ=255)

                                     tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                          >  1:589113/1‑139 (MQ=255)

                                      ccAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                         <  2:938768/139‑1 (MQ=255)

                                        aGCTCGCAAAACTCTACGCCCGACACAAACCTGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                       <  2:951370/139‑1 (MQ=255)

                                        aGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                       <  2:964181/139‑1 (MQ=255)

                                         gCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAct                                                                                      <  1:503560/139‑1 (MQ=255)

                                          cTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCActc                                                                                     >  2:540118/1‑139 (MQ=255)

                                                    tctACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTa                                                                               <  2:584491/135‑1 (MQ=255)

                                                    tctACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTa                                                                               >  1:584491/1‑135 (MQ=255)

                                                                         tgGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               >  1:385116/1‑66 (MQ=255)

                                                                         tgGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                               <  2:385116/66‑1 (MQ=255)

                                                                         tgGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCgg                                                        <  2:165415/137‑1 (MQ=255)

                                                                         tgGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCgg                                                        >  1:165415/1‑137 (MQ=255)

                                                                                gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCt                                                                                           <  1:867231/95‑1 (MQ=255)

                                                                                gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCt                                                                                           >  2:867231/1‑95 (MQ=255)

                                                                                gtctgCTTCGCGCCGCAGCCCACTTACTCACCC‑AAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCg                                                   <  1:962295/134‑1 (MQ=255)

                                                                                gtctgCTTCGCGCCGCAGCCCACTTACTCACCC‑AAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCg                                                   >  2:962295/1‑134 (MQ=255)

                                                                                 tctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                         >  1:703909/1‑96 (MQ=255)

                                                                                 tctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                         <  2:703909/96‑1 (MQ=255)

                                                                                       tCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGACTGTTGCTGGGCTTAGCGGCTGCGATCAACAagag                                        >  1:686426/1‑139 (MQ=255)

                                                                                          cgccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAAt                                     >  2:997097/1‑139 (MQ=255)

                                                                                                       ttACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAg                        <  2:976696/139‑1 (MQ=255)

                                                                                                            cACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGaa                   >  1:862311/1‑139 (MQ=255)

                                                                                                                  aaaaaTGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATacgac             <  1:997097/139‑1 (MQ=255)

                                                                                                                   aaaaTGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATacgacg            <  2:686426/139‑1 (MQ=255)

                                                                                                                         aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCaa                           <  2:744058/118‑1 (MQ=255)

                                                                                                                         aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCaa                           >  1:744058/1‑118 (MQ=255)

                                                                                                                         aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACttt      >  1:27274/1‑139 (MQ=255)

                                                                                                                             aTAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTTCGaa  <  2:929946/139‑1 (MQ=255)

                                                                                                                             aTAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTTCGaa  <  2:589113/139‑1 (MQ=255)

CAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTTCGAA  >  NC_000913/1833983‑1834246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A