Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,834,111	G→A	237 (TAG→TAA)	ydjX →	TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,834,111	0	G	A	100.0%	123.5 / NA	40	237 (TAG→TAA)	ydjX	TVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/17); total (23/17)

TTATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTT  >  NC_000913/1833975‑1834241

ttATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATaaaa                                                                                                                                  <  1:170484/139‑1 (MQ=255)

   ttttACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGa                                                                                                                               <  2:71613/139‑1 (MQ=255)

        cAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg                                                                                                                          <  2:49152/139‑1 (MQ=255)

           ctctGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCaa                                                                                                                       >  1:34794/1‑139 (MQ=255)

           ctctGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCaa                                                                                                                       >  2:655935/1‑139 (MQ=255)

             ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                     <  1:198668/139‑1 (MQ=255)

             ctgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACa                                                                                                                     <  2:83295/139‑1 (MQ=255)

              tgtctgGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACat                                                                                                                    >  1:445767/1‑139 (MQ=255)

                    gCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCa                                                                                                              <  1:655935/139‑1 (MQ=255)

                      gggCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAgt                                                                                                            >  2:33665/1‑139 (MQ=255)

                          cTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCa                                                                                                        >  2:505908/1‑139 (MQ=255)

                          cTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCa                                                                                                        >  1:175609/1‑139 (MQ=255)

                          cTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATCCAGTGTCa                                                                                                        >  2:107621/1‑139 (MQ=255)

                            ggCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATg                                                                                                      <  1:107621/139‑1 (MQ=255)

                                      aTTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGa                                                                                            <  2:34794/139‑1 (MQ=255)

                                       ttCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc                                                                                           <  1:505908/139‑1 (MQ=255)

                                             tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAATGATGTTGCAACATTATTCAGTGTCATg                                                                                                      >  2:762356/1‑122 (MQ=255)

                                             tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAATGATGTTGCAACATTATTCAGTGTCATg                                                                                                      <  1:762356/122‑1 (MQ=255)

                                             tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                     >  1:640475/1‑139 (MQ=255)

                                             tCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTg                                                                                     >  2:662593/1‑139 (MQ=255)

                                              ccAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                    <  2:688986/139‑1 (MQ=255)

                                               cAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCa                                                                                   <  1:193726/139‑1 (MQ=255)

                                                  cTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCActc                                                                                <  1:33665/139‑1 (MQ=255)

                                                                     gACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTg                                                             >  2:527992/1‑139 (MQ=255)

                                                                                gtgGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGc                                                  >  2:269717/1‑139 (MQ=255)

                                                                                    aTCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCg                                              <  2:640475/139‑1 (MQ=255)

                                                                                      ctgtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGAt                                            >  2:678184/1‑139 (MQ=255)

                                                                                      ctgtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGAt                                            >  2:423821/1‑139 (MQ=255)

                                                                                        gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                    <  1:498751/97‑1 (MQ=255)

                                                                                        gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATca                                          >  1:260245/1‑139 (MQ=255)

                                                                                        gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATACAGTGTCATGGAAAAAAGGACTGGCTGc                                                                                    >  2:498751/1‑97 (MQ=255)

                                                                                             cTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAag                                     >  2:169814/1‑139 (MQ=255)

                                                                                                cgcgCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAgaga                                  >  2:789133/1‑139 (MQ=255)

                                                                                                cgcgCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAgaga                                  >  1:234893/1‑139 (MQ=255)

                                                                                                 gcgcCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGCTGTTGCa                                                                                                                        <  2:492206/52‑1 (MQ=255)

                                                                                                 gcgcCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCa                                                                                                                        >  1:492206/1‑52 (MQ=255)

                                                                                                  cgccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                  <  1:92400/89‑1 (MQ=255)

                                                                                                  cgccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc                                                                                  >  2:92400/1‑89 (MQ=255)

                                                                                                   gccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTg                                                <  2:240342/122‑1 (MQ=255)

                                                                                                   gccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTg                                                >  1:240342/1‑122 (MQ=255)

                                                                                                                                gAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACtt  >  1:675303/1‑139 (MQ=255)

TTATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTT  >  NC_000913/1833975‑1834241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A