Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,834,111 |
G→A |
*237* (TAG→TAA) |
ydjX → |
TVP38/TMEM64 family inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,834,111 | 0 | G | A | 100.0%
| 112.5
/ NA
| 36 | *237* (TAG→TAA) | ydjX | TVP38/TMEM64 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/17); total (19/17) |
TATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTT > NC_000913/1833976‑1834242
|
tATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAAc > 2:164066/1‑139 (MQ=255)
ctctGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCaa > 2:409668/1‑139 (MQ=255)
cGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAg > 1:133039/1‑139 (MQ=255)
ggCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg > 2:861402/1‑119 (MQ=255)
ggCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg < 1:861402/119‑1 (MQ=255)
cgcTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGa > 1:246322/1‑112 (MQ=255)
cgcTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGa < 2:246322/112‑1 (MQ=255)
cTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCActc > 1:207282/1‑139 (MQ=255)
tCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCActct < 1:107531/139‑1 (MQ=255)
gCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGt < 1:18055/139‑1 (MQ=255)
aCGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGc < 1:210436/137‑1 (MQ=255)
aCGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGc > 2:210436/1‑137 (MQ=255)
aCGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTg > 2:318033/1‑139 (MQ=255)
cGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg < 2:928419/83‑1 (MQ=255)
cGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTg > 1:928419/1‑83 (MQ=255)
cacaAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTggg < 1:318033/139‑1 (MQ=255)
tgGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc < 2:330434/104‑1 (MQ=255)
tgGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGc > 1:330434/1‑104 (MQ=255)
ggATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTg < 2:219544/139‑1 (MQ=255)
tgtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATc > 1:7670/1‑139 (MQ=255)
gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc < 2:383482/90‑1 (MQ=255)
gtctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc > 1:383482/1‑90 (MQ=255)
tctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc < 2:363718/98‑1 (MQ=255)
tctgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCAc > 1:363718/1‑98 (MQ=255)
tgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc < 1:847415/87‑1 (MQ=255)
tgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGAc > 2:847415/1‑87 (MQ=255)
tgCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATcaaca > 1:489489/1‑139 (MQ=255)
cgcgCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAgaga > 1:717087/1‑139 (MQ=255)
cgccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGTAAAAAAGGACTGGCTGCACTCTGTTTACTGGc < 2:551797/102‑1 (MQ=255)
cgccgcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGAGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGc > 1:551797/1‑102 (MQ=255)
gcAGCCCACTTACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATgcgg < 1:261158/139‑1 (MQ=255)
ttACTCACCCAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGTTCAACAAGAGAATGCGGCGGCAAAAg < 2:133039/139‑1 (MQ=255)
ccAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTAc < 1:877756/77‑1 (MQ=255)
ccAAAAAATGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTAc > 2:877756/1‑77 (MQ=255)
aaaaTGAAGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATacgacg < 1:164066/139‑1 (MQ=255)
aaGGATAAAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTCGCGGCTGTGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACttt > 2:860430/1‑139 (MQ=255)
|
TATTTTACAACTCTGTCTGGCGGGCCTGGCGCTGTTTATTCTCGTCCAGCTCGCAAAACTCTACGCCCGACACAAACATGTGGATCTGTCTGCTTCGCGCCGCAGCCCACTTACTCACCCAAAAAATGAAGGATAGAACGATGTTGCAACATTATTCAGTGTCATGGAAAAAAGGACTGGCTGCACTCTGTTTACTGGCTGTTGCTGGGCTTAGCGGCTGCGATCAACAAGAGAATGCGGCGGCAAAAGTGGAATACGACGGACTTT > NC_000913/1833976‑1834242
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
