Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,325	A→G	T104T (ACT→ACC)	yeaE ←	aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,325	0	A	G	100.0%	117.7 / NA	33	T104T (ACT→ACC)	yeaE	aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/17); total (16/17)

AGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCG  >  NC_000913/1865196‑1865457

aGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgaga                                                                                                                             <  1:324940/139‑1 (MQ=255)

            gTTAGAAATGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTg                                                                                                                 >  2:486368/1‑139 (MQ=255)

             ttAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              <  2:736570/125‑1 (MQ=255)

             ttAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              >  1:736570/1‑125 (MQ=255)

                gAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAGGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              >  2:95774/1‑122 (MQ=255)

                gAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgag                                                                                                                              <  1:95774/122‑1 (MQ=255)

                     gCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCgcat                                                                                                        >  1:497007/1‑139 (MQ=255)

                          aGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCAtt                                                                                                   >  1:176774/1‑139 (MQ=255)

                                              gCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAg                                                                               <  2:732683/139‑1 (MQ=255)

                                                         ttCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGa                                                                    <  1:486368/139‑1 (MQ=255)

                                                          tCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGAt                                                                   <  2:114549/139‑1 (MQ=255)

                                                           ccATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  <  2:497007/139‑1 (MQ=255)

                                                           ccATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  <  2:176774/139‑1 (MQ=255)

                                                                    cGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTaga                                                         >  1:943211/1‑139 (MQ=255)

                                                                     gACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTagag                                                        <  2:394326/139‑1 (MQ=255)

                                                                          tctcTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTcc                                                                         <  1:525175/117‑1 (MQ=255)

                                                                          tctcTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTcc                                                                         >  2:525175/1‑117 (MQ=255)

                                                                                                       tgtAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAgaga                                                       >  1:304155/1‑106 (MQ=255)

                                                                                                       tgtAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAgaga                                                       <  2:304155/106‑1 (MQ=255)

                                                                                                                 aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              >  1:37318/1‑105 (MQ=255)

                                                                                                                 aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              <  2:37318/105‑1 (MQ=255)

                                                                                                                 aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTCATGCTTCCCCAac            >  1:1088566/1‑139 (MQ=255)

                                                                                                                 aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCtt                   <  2:411390/132‑1 (MQ=255)

                                                                                                                 aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCtt                   >  1:411390/1‑132 (MQ=255)

                                                                                                                 aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAac            >  2:651636/1‑139 (MQ=255)

                                                                                                                   aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  <  2:333496/83‑1 (MQ=255)

                                                                                                                   aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                  >  1:333496/1‑83 (MQ=255)

                                                                                                                   aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              >  2:677539/1‑103 (MQ=255)

                                                                                                                   aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGa                                              <  1:677539/103‑1 (MQ=255)

                                                                                                                          gaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGa                                >  1:765537/1‑110 (MQ=255)

                                                                                                                          gaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGa                                <  2:765537/110‑1 (MQ=255)

                                                                                                                          gaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTc   >  2:1073818/1‑139 (MQ=255)

                                                                                                                           aTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCg  <  1:651636/139‑1 (MQ=255)

AGCATAATCAAGGTTAGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCG  >  NC_000913/1865196‑1865457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A