Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 765 46.1 2269152 93.8% 2128464 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,782,393 (C)5→4 coding (334/4581 nt) ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,3890C.100.0% 267.0 / NA 63coding (338/4581 nt)ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (30/33);  total (30/33)

TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAA  >  NC_000913/2782252‑2782521
                                                                                                                                         |                                                                                                                                    
tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                     <  2:415439/139‑1 (MQ=255)
        tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATg                                                                                                                            >  1:890282/1‑139 (MQ=255)
         cAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                               <  2:470086/136‑5 (MQ=255)
         cAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccccgtca                                                                                                                               >  1:470086/1‑132 (MQ=255)
            tGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAAtt                                                                                                                        >  1:825117/1‑139 (MQ=255)
              aGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGa                                                                                                                      <  2:847717/139‑1 (MQ=255)
                tATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACt                                                                                                                    <  2:825117/139‑1 (MQ=255)
                   tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTc                                                                                                                 >  1:184791/1‑139 (MQ=255)
                     cATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAACCATACTGATTGCCACTCTCGATTGTCATACCTGTGGAAATA‑CCCCTTCATGAATTGactgactg                                                                                                               >  2:611810/1‑139 (MQ=255)
                       ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGcc                                                                                                             >  2:689724/1‑139 (MQ=255)
                       ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGcc                                                                                                             <  2:85403/139‑1 (MQ=255)
                          gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCAcc                                                                                                          >  1:147505/1‑139 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  2:890282/139‑1 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:363664/139‑1 (MQ=255)
                               ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:689724/139‑1 (MQ=255)
                                     ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  2:184791/139‑1 (MQ=255)
                                     ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  2:486822/139‑1 (MQ=255)
                                     ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:245065/139‑1 (MQ=255)
                                          cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                          >  2:791810/1‑139 (MQ=255)
                                              tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                     <  1:837883/93‑1 (MQ=255)
                                              tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAcc                                                                                                                                     >  2:837883/1‑93 (MQ=255)
                                              tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAg                                                                                      >  2:337869/1‑139 (MQ=255)
                                                aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                    >  1:860565/1‑139 (MQ=255)
                                                aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                    >  2:72805/1‑139 (MQ=255)
                                                aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                    >  2:643989/1‑139 (MQ=255)
                                                       gCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             <  1:72805/139‑1 (MQ=255)
                                                           ttAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaa                                                                         >  1:586804/1‑139 (MQ=255)
                                                             aaTTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               >  2:360199/1‑115 (MQ=255)
                                                             aaTTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:360199/115‑1 (MQ=255)
                                                                  tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:438849/110‑1 (MQ=255)
                                                                  tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               >  2:438849/1‑110 (MQ=255)
                                                                  tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  >  1:363186/1‑139 (MQ=255)
                                                                  tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  <  1:643989/139‑1 (MQ=255)
                                                                   cGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                 <  1:840642/139‑1 (MQ=255)
                                                                     ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                               <  2:465737/139‑1 (MQ=255)
                                                                      tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                              <  1:337869/139‑1 (MQ=255)
                                                                         aTTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTc                                                           <  2:363186/139‑1 (MQ=255)
                                                                           tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                         <  2:860565/139‑1 (MQ=255)
                                                                           tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                         <  1:791810/139‑1 (MQ=255)
                                                                           tGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCt                                                         <  1:656599/139‑1 (MQ=255)
                                                                             cTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGGCTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCtat                                                       <  1:611810/139‑1 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     <  1:630618/83‑1 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               <  1:462871/89‑1 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                               >  2:462871/1‑89 (MQ=255)
                                                                                       cTTTATAAACGTCCTGATAGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                     >  2:630618/1‑83 (MQ=255)
                                                                                                cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACt                                                                                                                    <  1:803982/59‑1 (MQ=255)
                                                                                                cGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCAGTCATGAATTGACt                                                                                                                    >  2:803982/1‑59 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CTCCGTCATGAATTGACTGTCTGCCa                                                                                                            <  2:769143/65‑1 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             >  1:936603/1‑96 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                             <  2:936603/96‑1 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGAtt                                   >  2:5251/1‑138 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGAtt                                   <  1:5251/138‑1 (MQ=255)
                                                                                                  tCCTGATTGCCACTCTCGATAGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCa                                                                                                            >  1:769143/1‑65 (MQ=255)
                                                                                                   ccTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  >  2:1003657/1‑106 (MQ=255)
                                                                                                   ccTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                  <  1:1003657/106‑1 (MQ=255)
                                                                                                      gATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTa                                                                                                      <  2:89523/67‑1 (MQ=255)
                                                                                                      gATTGCCACTCTCGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTa                                                                                                      >  1:89523/1‑67 (MQ=255)
                                                                                                                 tcGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATccac                                         <  2:1131955/117‑1 (MQ=255)
                                                                                                                 tcGATTGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATccac                                         >  1:1131955/1‑117 (MQ=255)
                                                                                                                      tGTCGTACCTGTGGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATa              >  1:1101868/1‑139 (MQ=255)
                                                                                                                                 tgGAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGa   >  2:10034/1‑139 (MQ=255)
                                                                                                                                  ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATg                                                                                                   >  1:668577/1‑42 (MQ=255)
                                                                                                                                  ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     >  1:1021580/1‑104 (MQ=255)
                                                                                                                                  ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                     <  2:1021580/104‑1 (MQ=255)
                                                                                                                                  ggAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa  >  1:402566/1‑139 (MQ=255)
                                                                                                                                  ggAAATA‑CCCCGTCATGAATTCACTGTCTGCCACCGTTAATg                                                                                                   <  2:668577/42‑1 (MQ=255)
                                                                                                                                   gAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtat                                                                                            >  1:372657/1‑48 (MQ=255)
                                                                                                                                   gAAATA‑CCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtat                                                                                            <  2:372657/48‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
TACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAA  >  NC_000913/2782252‑2782521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A