Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
760 |
67.6 |
3323148 |
92.7% |
3080558 |
109.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,713,568 |
T→C |
W225R (TGG→CGG) |
srmB → |
ATP‑dependent RNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,713,568 | 0 | T | C | 100.0%
| 156.2
/ NA
| 44 | W225R (TGG→CGG) | srmB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/23); total (21/23) |
AACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCT > NC_000913/2713440‑2713686
|
aaCAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTGAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcg < 1:389699/139‑1 (MQ=255)
aCAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcgc < 2:351880/139‑1 (MQ=255)
aGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCgcgc < 2:297008/139‑1 (MQ=255)
aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg > 2:1387536/1‑139 (MQ=255)
aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg > 2:1013346/1‑139 (MQ=255)
aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg > 2:240380/1‑139 (MQ=255)
tttCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATcctgt < 2:1625357/139‑5 (MQ=255)
cGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAAc > 1:96275/1‑139 (MQ=255)
cTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcg < 1:251354/139‑1 (MQ=255)
ggAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg < 2:1276554/139‑1 (MQ=255)
ggCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGc < 2:183702/139‑1 (MQ=255)
cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATaa > 2:903881/1‑126 (MQ=255)
cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATaa < 1:903881/126‑1 (MQ=255)
cgatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTg > 2:1365246/1‑139 (MQ=255)
gatgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTgg > 1:1286429/1‑139 (MQ=255)
tgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTg > 2:1408706/1‑139 (MQ=255)
cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa < 2:710083/139‑1 (MQ=255)
cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa < 1:1387536/139‑1 (MQ=255)
tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg > 1:371887/1‑139 (MQ=255)
tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg > 2:876023/1‑139 (MQ=255)
ctgGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGAccc < 2:1280166/139‑1 (MQ=255)
tCCGGTGGAAGTTTCTGCCAATCCCTCCTCCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAAtt < 1:1013346/139‑1 (MQ=255)
tGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt > 1:14755/1‑74 (MQ=255)
tGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt < 2:14755/74‑1 (MQ=255)
gTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGc < 1:166212/139‑1 (MQ=255)
tttCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCg < 2:1130634/116‑1 (MQ=255)
tttCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCg > 1:1130634/1‑116 (MQ=255)
tttCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCg < 2:1209801/139‑1 (MQ=255)
ttCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt < 1:240380/139‑1 (MQ=255)
tGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAag > 1:682116/1‑139 (MQ=255)
cAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgt < 2:96275/139‑1 (MQ=255)
tCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtgagcgtg > 1:586576/1‑139 (MQ=255)
gTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt < 2:1203663/47‑1 (MQ=255)
gTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt > 1:1203663/1‑47 (MQ=255)
tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcg > 1:652444/1‑59 (MQ=255)
tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcg < 2:652444/59‑1 (MQ=255)
tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa < 2:1133401/72‑1 (MQ=255)
tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa > 1:1133401/1‑72 (MQ=255)
aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa > 2:1229600/1‑70 (MQ=255)
aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa < 1:1229600/70‑1 (MQ=255)
aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCt > 2:285952/1‑139 (MQ=255)
aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCt > 1:1544040/1‑139 (MQ=255)
atcaGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCg < 1:884923/82‑1 (MQ=255)
atcaGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCg > 2:884923/1‑82 (MQ=255)
|
AACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCT > NC_000913/2713440‑2713686
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
