Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,713,568	T→C	W225R (TGG→CGG)	srmB →	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,713,568	0	T	C	100.0%	76.8 / NA	23	W225R (TGG→CGG)	srmB	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/13); total (10/13)

AACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAAC  >  NC_000913/2713440‑2713693

aaCAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcg                                                                                                                     <  2:332494/139‑1 (MQ=255)

     aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                                >  2:11635/1‑139 (MQ=255)

          gCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCgatgat                                                                                                           >  2:571705/1‑139 (MQ=255)

                            ggAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACcgcg                                                                                         <  1:394567/139‑1 (MQ=255)

                                     tgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTg                                                                                <  1:11635/139‑1 (MQ=255)

                                     tgcgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTg                                                                                >  1:355467/1‑139 (MQ=255)

                                       cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  1:481459/139‑1 (MQ=255)

                                       cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  1:571705/139‑1 (MQ=255)

                                       cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                              <  1:896050/139‑1 (MQ=255)

                                                    tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg                                                                 >  2:339721/1‑139 (MQ=255)

                                                     gCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGc                                                                <  1:339721/139‑1 (MQ=255)

                                                             tCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCg                                                        <  2:355467/139‑1 (MQ=255)

                                                                         tCCGGTGGAAGTTTCTGCTAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAAtt                                            >  2:517198/1‑139 (MQ=255)

                                                                                     ttCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                                <  1:517198/139‑1 (MQ=255)

                                                                                               cccTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtgagc                      <  2:510912/139‑1 (MQ=255)

                                                                                                              cgcAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTgg       >  1:742974/1‑139 (MQ=255)

                                                                                                                cAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCg                                                              <  1:878639/82‑1 (MQ=255)

                                                                                                                cAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCg                                                              >  2:878639/1‑82 (MQ=255)

                                                                                                                 aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTg                                                                                >  1:750638/1‑63 (MQ=255)

                                                                                                                 aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTg                                                                                <  2:750638/63‑1 (MQ=255)

                                                                                                                   aaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAAc  >  2:42017/1‑139 (MQ=255)

                                                                                                                        ttCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCt                                                 <  1:567518/87‑1 (MQ=255)

                                                                                                                        ttCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCt                                                 >  2:567518/1‑87 (MQ=255)

AACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAAC  >  NC_000913/2713440‑2713693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A